Alcohol Causes Lasting Differential Transcription in Drosophila Mushroom Body Neurons.

Genetics
Emily PetruccelliKarla R Kaun

Abstract

Repeated alcohol experiences can produce long-lasting memories for sensory cues associated with intoxication. These memories can problematically trigger relapse in individuals recovering from alcohol use disorder (AUD). The molecular mechanisms by which ethanol changes memories to become long-lasting and inflexible remain unclear. New methods to analyze gene expression within precise neuronal cell types can provide further insight toward AUD prevention and treatment. Here, we used genetic tools in Drosophila melanogaster to investigate the lasting consequences of ethanol on transcription in memory-encoding neurons. Drosophila rely on mushroom body (MB) neurons to make associative memories, including memories of ethanol-associated sensory cues. Differential expression analyses revealed that distinct transcripts, but not genes, in the MB were associated with experiencing ethanol alone compared to forming a memory of an odor cue associated with ethanol. Adult MB-specific knockdown of spliceosome-associated proteins demonstrated the necessity of RNA-processing in ethanol memory formation. These findings highlight the dynamic, context-specific regulation of transcription in cue-encoding neurons, and the lasting effect of ethanol on ...Continue Reading

Associated Datasets

References

Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·C H BaileyE R Kandel
Feb 19, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sean E McGuireRonald L Davis
Sep 13, 2007·Nature Reviews. Neuroscience·Clive R Bramham, David G Wells
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Dec 17, 2009·Current Biology : CB·Anita V Devineni, Ulrike Heberlein
Dec 2, 2010·Biochemical Society Transactions·Joel D Richter
Apr 19, 2011·Nature Neuroscience·Karla R KaunUlrike Heberlein
Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tijana CopfThomas Preat
Jul 30, 2011·Alcoholism, Clinical and Experimental Research·Harish R KrishnanNigel S Atkinson
Nov 1, 2011·Cell·K G GuruharshaSpyros Artavanis-Tsakonas
Aug 3, 2012·Nucleic Acids Research·Gilbert L HenrySean R Eddy
Oct 16, 2012·Cell Reports·Arnim JenettChristopher T Zugates
Nov 12, 2013·Biological Psychiatry·Changhoon LeeR Adron Harris
Nov 12, 2013·Neuroscience and Biobehavioral Reviews·Agnes J JasinskaYavor Yalachkov
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
May 29, 2014·Trends in Pharmacological Sciences·James R TrudellR Adron Harris
Aug 31, 2014·International Review of Neurobiology·Sean P Farris, R Dayne Mayfield
Dec 6, 2014·Cold Spring Harbor Perspectives in Biology·Cristina M Alberini, Eric R Kandel
Jan 30, 2015·Wiley Interdisciplinary Reviews. RNA·Benjamin Cieply, Russ P Carstens
Mar 10, 2015·Nature Biotechnology·Alyssa C FrazeeJeffrey T Leek
Oct 6, 2015·Addiction Biology·Kelly E CourtneyLara A Ray
Nov 1, 2016·Journal of Neurochemistry·J David Sweatt
Feb 29, 2016·Addictive Behaviors·Martine GroefsemaMaartje Luijten
Apr 20, 2016·Nature Communications·Benjamin LacarFred H Gage
Jun 21, 2016·Cell·Toshiaki ShigeokaChristine E Holt
Jul 23, 2016·Nature Reviews. Neuroscience·Dorit Ron, Segev Barak
Jul 28, 2016·Neurobiology of Learning and Memory·Shane G PoplawskiTed Abel
Aug 26, 2016·Nature Protocols·Mihaela PerteaSteven L Salzberg
Sep 30, 2016·PloS One·Katarina Pankova, Alexander Borst
May 6, 2017·Behavioural Processes·Milan D ValyearNadia Chaudhri
Jul 25, 2017·Neuroscience·Shusaku Uchida, Gleb P Shumyatsky
Nov 14, 2017·Progress in Neuro-psychopharmacology & Biological Psychiatry·Kelly J Clemens, Nathan M Holmes

❮ Previous
Next ❯

Citations

Apr 17, 2021·Frontiers in Behavioral Neuroscience·Camilla RoselliIsaac Cervantes-Sandoval

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE108525

Methods Mentioned

BETA
RNA-seq
FACS
Hi-Seq
PCR

Software Mentioned

RStudio
DAVID
UpSetR
hisat2
Samtools
GENEWIZ
FIJI
Ballgown
INTACT
NIS

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.

Related Papers

BioRxiv : the Preprint Server for Biology
Emily PetruccelliKarla R Kaun
Issues in Mental Health Nursing
Jacquelyn H Flaskerud
MLO: Medical Laboratory Observer
Ronald H Laessig
European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
Irène Zintel
© 2021 Meta ULC. All rights reserved