Alcohol dehydrogenase (ADH) in yeasts. II. NAD+-and NADP+-dependent alcohol dehydrogenases in Saccharomycopsis lipolytica

Zeitschrift für allgemeine Mikrobiologie
G Barth, W Künkel

Abstract

In Sm. lipolytica one NAD+-dependent and three NADP+-dependent alcohol dehydrogenases are detectable by polyacrylamide gelelectrophoresis. The NAD+-dependent ADH (ADH I), with a molecular weight of 240,000 daltons, reacts more intensively with long-chain alcohols (octanol) than with short-chain alcohols (methanol, ethanol). The ADH I is not or only minimally subject to glucose repression. Besides the ADH I band no additional inducible NAD+-dependent ADH band is gel-electrophoretically detectable during growth of yeast cells in medium containing ethanol or paraffin. The ADH I band is very probably formed by two ADH enzymes with the same electrophoretic mobility. The NADP+-dependent alcohol dehydrogenases (ADH II--IV) react with methanol, ethanol and octanol with different intensity. In polyacrylamide gradients two bands of NADP+-dependent ADH are detectable: one with a molecular weight of 70,000 daltons and the other with 120,000 daltons. The occurrence of the three NADP+-dependent alcohol dehydrogenases is regulated by the carbon source of the medium. Sm. lipolytica shows a high tolerance against allylalcohol. Resistant mutants can be isolated only at concentrations of 1 M allylalcohol in the medium. All isolates of allylalcoho...Continue Reading

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Citations

Oct 1, 1986·Biochemical Genetics·R BrzezinskiJ P Thirion
Jan 1, 1983·Zeitschrift für allgemeine Mikrobiologie·G Barth, H Weber
Jan 1, 1984·Zeitschrift für allgemeine Mikrobiologie·G Barth, H Weber
Apr 1, 1997·FEMS Microbiology Reviews·G Barth, C Gaillardin
Jan 1, 1985·Antonie van Leeuwenhoek·G Barth, H Weber
Nov 13, 2019·Applied Microbiology and Biotechnology·Svetlana V Kamzolova, Igor G Morgunov
Sep 9, 2000·FEMS Microbiology Reviews·C L FloresC Gancedo

Related Concepts

Acetic Acids
Alcohol Oxidoreductases
Ethanol
Cochliobolus
Enzyme Repression
Glucose, (beta-D)-Isomer
Alloenzymes
NADH
NADP
Saccharomycopsis

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