Alcohol dehydrogenase and ethanol tolerance at the cellular level in Drosophila melanogaster

The Journal of Experimental Zoology
B W GeerL J Shanner

Abstract

Exposure of early third instar larvae of Drosophila melanogaster to a nonlethal dose of ethanol was detrimental to larvae lacking alcohol dehydrogenase (ADH) but beneficial to wild-type larvae in terms of surviving a later ethanol tolerance test, indicating that one of the important functions of the ADH system is to supply derivatives of ethanol to larvae that in turn promote ethanol tolerance. High intracellular concentrations of ethanol in ADH-deficient (Adhn2) larvae fed ethanol were accompanied by a decrease in the cell membrane infoldings of fat body cells, suggesting that the capacities to absorb and release molecules were reduced. Marked effects of ethanol on the endoplasmic reticulum and mitochondria of ADH-deficient larvae were also evident. The absence of similar changes in wild-type larvae that were fed moderate levels of ethanol showed that the ADH system kept the intracellular level of ethanol at a concentration low enough to avoid cell damage. A cytometric analysis of electron micrographs showed that there were ethanol-induced reductions in glycogen, lipid, and protein stores in the fat body cells of ADH-deficient larvae fed 1.25% ethanol (v/v) compared with null larvae fed an ethanol-free diet. This finding impli...Continue Reading

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Citations

Apr 1, 1993·Comparative Biochemistry and Physiology. Comparative Physiology·R R MillerB W Geer
Jun 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·B W GeerS W McKechnie
May 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·M L MartelB W Geer
Feb 19, 2011·Chemico-biological Interactions·Aída Hernández-TobíasHéctor Riveros-Rosas
Apr 11, 2012·Alcoholism, Clinical and Experimental Research·Joseph T AlaimoJill C Bettinger
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Jun 12, 2021·Scientific Reports·Isabell SchumannAndreas S Thum
Jun 14, 2021·Alcoholism, Clinical and Experimental Research·Michelle BonillaMariano Loza-Coll

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