Alcohol reduces MMP-2 in humans and isolated smooth muscle cells

Alcohol
Nicola FiottiCarlo Giansante

Abstract

Alcoholic beverages are known to exert a protective effect on atherosclerotic disease. This study aimed to assess the in vivo and in vitro effects of alcohol on matrix metalloproteinase (MMP) -2 and -9, known to determine atherosclerosis progression. Eighteen healthy volunteers, regular drinkers (two standard alcohol servings/day, on average) at first examination (baseline) were asked to abstain from any alcoholic beverage for one week (abstention), and then to assume two standard alcohol servings of beer daily for 1 week (re-exposure). Activity of MMP-2 and -9, total antioxidant activity (AOA), glutathione (GSH) plasma levels were carried out at baseline, at the end of abstention, and after 1 week of re-exposure. To validate the in vivo results, MMP-2 activity and expression, AOA, and GSH, were determined in human smooth muscle cells treated for 96 h with increasing concentrations (12.5-100 mM) of ethanol. MMP-2, but not MMP-9 plasma activity was higher at abstention than at baseline or re-exposure (P<.001 and P< or =.005, respectively). Changes in AOA and GSH throughout the study were not significant. No correlation was found between MMPs and antioxidant activity. In vitro, ethanol at 25 mM reduced by around 10% MMP-2 activit...Continue Reading

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Citations

Nov 26, 2009·Clinical and Experimental Pharmacology & Physiology·Helena C ParkingtonMarianne Tare
May 19, 2012·Nutrients·Paul A Cahill, Eileen M Redmond
Nov 8, 2011·Journal of the American College of Surgeons·Alfredo C CordovaBauer E Sumpio
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Aug 11, 2015·Analytical Cellular Pathology (Amsterdam)·Beatriz Aparecida Fioruci-FontanelliFrancisco Eduardo Martinez

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