Alcoholic Liver Disease: A Mouse Model Reveals Protection by Lactobacillus fermentum

Clinical and Translational Gastroenterology
Rosario BaroneAntonella Marino Gammazza

Abstract

Alcoholism is one of the most devastating diseases with high incidence, but knowledge of its pathology and treatment is still plagued with gaps mostly because of the inherent limitations of research with patients. We developed an animal model for studying liver histopathology, Hsp (heat-shock protein)-chaperones involvement, and response to treatment. The system was standardized using mice to which ethanol was orally administered alone or in combination with Lactobacillus fermentum following a precise schedule over time and applying, at predetermined intervals, a battery of techniques (histology, immunohistochemistry, western blotting, real-time PCR, immunoprecipitation, 3-nitrotyrosine labeling) to assess liver pathology (e.g., steatosis, fibrosis), and Hsp60 and iNOS (inducible form of nitric oxide synthase) gene expression and protein levels, and post-translational modifications. Typical ethanol-induced liver pathology occurred and the effect of the probiotic could be reliably monitored. Steatosis score, iNOS levels, and nitrated proteins (e.g., Hsp60) decreased after probiotic intake. We describe a mouse model useful for studying liver disease induced by chronic ethanol intake and for testing pertinent therapeutic agents, e...Continue Reading

References

Jan 28, 1995·Lancet·S Sherlock
Feb 6, 1999·The American Journal of Gastroenterology·A KeshavarzianS Pethkar
Nov 23, 2000·Nature·T Finkel, N J Holbrook
Jun 8, 2001·Alcoholism, Clinical and Experimental Research·P de la M HallJ C Bode
Sep 1, 2001·Trends in Molecular Medicine·S StewartC P Day
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 19, 2002·Circulation·S R KirchhoffA A Knowlton
Dec 25, 2002·Free Radical Biology & Medicine·Carmela Loguercio, Alessandro Federico
Mar 4, 2003·Gastroenterology·Gavin E Arteel
Jun 11, 2003·Journal of Gastroenterology and Hepatology·Zoltán RakonczayTamás Takacs
Jun 28, 2003·Best Practice & Research. Clinical Gastroenterology·Christiane Bode, J Christian Bode
Aug 26, 2003·The American Journal of Pathology·Zhanxiang ZhouY James Kang
Mar 9, 2004·Biochemical and Biophysical Research Communications·Mariko Murata, Shosuke Kawanishi
Mar 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rafael Radi
Sep 7, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Aparna VenkatramanVictor M Darley-Usmar
Feb 11, 2005·Current Gastroenterology Reports·Shane J Mills, Stephen A Harrison
Feb 19, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·S BasuroyR K Rao
Sep 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Qing-Gao DengHidekazu Tsukamoto
Oct 24, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kwan-Hoon MoonByoung J Song
Jan 9, 2007·Inflammatory Bowel Diseases·Julia-Stefanie FrickIngo B Autenrieth
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Dec 29, 2007·Alcoholism, Clinical and Experimental Research·Yueming TangAli Keshavarzian
Mar 29, 2008·Methods in Molecular Biology·Gavin E Arteel
Jun 28, 2008·Scandinavian Journal of Gastroenterology·Eduardo Garcia VilelaAloísio Sales Da Cunha
Dec 26, 2008·European Journal of Histochemistry : EJH·C CampanellaF Cappello
Apr 30, 2009·Archives of Biochemistry and Biophysics·Thomas KoeckKulwant S Aulak
Aug 4, 2009·Alcoholism, Clinical and Experimental Research·Ece MutluPatrick Gillevet
Sep 22, 2009·Immunity·Breck A DuerkopLora V Hooper
Mar 20, 2010·World Journal of Gastroenterology : WJG·Gyongyi Szabo, Shashi Bala
Sep 2, 2010·World Journal of Gastroenterology : WJG·Sahar K Hegazy, Mohamed M El-Bedewy
Oct 6, 2011·Biochimica Et Biophysica Acta·Blake R ZelicksonVictor M Darley-Usmar
Dec 20, 2011·Biochimica Et Biophysica Acta·Marc FransenOksana Apanasets
Dec 27, 2011·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Federico SalamoneGiovanni Li Volti
Apr 14, 2012·International Journal of Hepatology·Aditya Ambade, Pranoti Mandrekar
Jun 30, 2012·Biochemistry Research International·Davide Degli EspostiAntoinette Lemoine
Jul 17, 2012·The American Journal of Pathology·Jacqueline C BouchardDaniel G Remick
Feb 13, 2013·Alcoholism, Clinical and Experimental Research·Rotonya M CarrRexford S Ahima
Feb 26, 2013·Nutrients·Filippo MacalusoValentina Di Felice
Apr 18, 2013·Journal of Tissue Engineering and Regenerative Medicine·Valentina Di FeliceGiovanni Zummo

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Citations

Mar 1, 2017·Journal of Cellular and Molecular Medicine·Rosario BaroneCristoforo Pomara
Jan 10, 2019·Nutrients·Sanjoy RoychowdhuryGail A M Cresci
Jun 5, 2019·International Journal of Molecular Sciences·Ling-Zu KongTaeho Kwon
Sep 16, 2019·Journal of Dairy Science·YoHan NamWonyong Kim
Nov 16, 2019·Critical Reviews in Food Science and Nutrition·Karim NaghmouchiDjamel Drider
Feb 26, 2019·Frontiers in Pharmacology·Francesco CappelloAlberto J L Macario
Nov 14, 2019·International Journal of Molecular Sciences·Abdullah Hoter, Hassan Y Naim
Dec 11, 2020·Gut Microbes·André A SantosCecília M P Rodrigues
Feb 3, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jose Alberto Molina-TijerasJulio Gálvez
Jan 1, 2021·International Journal of Molecular Sciences·Na Young Lee, Ki Tae Suk

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Methods Mentioned

BETA
protein folding
blood collection
PCR
electrophoresis
immunoprecipitation

Software Mentioned

ImageJ
GraphPad Prism

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