Aldo-keto reductases in the eye.

Journal of Ophthalmology
Shun Ping HuangJ Mark Petrash

Abstract

Aldose reductase (AKR1B1) is an NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway. Accelerated glucose metabolism through this pathway has been implicated in diabetic cataract and retinopathy. Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase gene superfamily. This opens the possibility that AKR1B10 may also contribute to diabetic complications. The goal of the current study was to characterize the expression profiles of AKR1B1 and AKR1B10 in the human eye. Using quantitative reverse transcriptase-PCR and immunohistochemical staining, we observed expression of both AKR genes in cornea, iris, ciliary body, lens, and retina. Expression of AKR1B1 was the highest in lens and retina, whereas AKR1B10 was the highest in cornea. Lenses from transgenic mice designed for overexpression of AKR1B10 were not significantly different from nontransgenic controls, although a significant number developed a focal defect in the anterior lens epithelium following 6 months of experimentally induced diabetes. However, lenses from AKR1B10 mice remained largely transparent following longterm diabetes. These results indicate that AKR1B1 and AKR1B10 may...Continue Reading

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Citations

Aug 27, 2013·Cell Stress & Chaperones·Nicholas ShawAndrea Hodgkinson
Nov 1, 2013·Drug Metabolism Reviews·Petra Malátková, Vladimír Wsól
Feb 19, 2011·Chemico-biological Interactions·Gregory J ZablockiJ Mark Petrash
Mar 5, 2013·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Yousef AldebasiAbdelmarouf Mohieldein
Dec 30, 2014·Chemico-biological Interactions·Anson SnowJ Mark Petrash
Apr 14, 2017·Vision Research·Alexander V Ljubimov
Dec 3, 2016·World Journal of Experimental Medicine·Alexander DontsovNatalia Sakina
May 31, 2014·Journal of the American Society of Nephrology : JASN·Miguel A LanaspaRichard J Johnson
Dec 14, 2018·Experimental and Therapeutic Medicine·Qiaoli Nie, Xiaoyan Zhang
Feb 2, 2021·Journal of Diabetes and Metabolic Disorders·Ambrose Teru PatrickWan Jin Jahng
Dec 18, 2020·Frontiers in Endocrinology·Ali A HajeyahValerie B O'Donnell
Jul 20, 2021·Archives of Pharmacal Research·Sapna ThakurMalkhey Verma

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Methods Mentioned

BETA
transgenic
PCR
electrophoresis
Protein Assay
column chromatography
nucleic acid hybridization
light scattering
light
dissection

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