PMID: 2493386Jan 1, 1989Paper

Aldose reductase and polyol in cultured pericytes of human retinal capillaries

Experimental Eye Research
T C HohmanW G Robison

Abstract

Aldose reductase has been found in several tissues involved in the various complications of long-term diabetes. Since the loss of intramural pericytes is typical of early microangiopathy of the diabetic retina, the presence of aldose reductase and its activity were investigated in pericytes cultured from capillaries of human retinas. Verification that contaminating cells had been removed was made on the basis of the pericytes' distinctive appearance in culture, inability to internalize acetylated-low-density lipoprotein, and immunoreactivity for muscle actin. Using pure cultures of human pericytes, the presence of aldose reductase was demonstrated immunohistochemically with antibodies directed against human placental aldose reductase, and aldose reductase activity was shown biochemically by monitoring the accumulating of xylitol in cells incubated with xylose. Xylitol accumulation was inhibited by the inclusion of an aldose reductase inhibitor in the xylose-containing medium.

References

Dec 5, 1975·Science·S M BuzneyW G Robison
Nov 1, 1986·Current Eye Research·Y AkagiJ H Kinoshita
Jul 1, 1985·The Journal of Cell Biology·I M Herman, P A D'Amore
Aug 1, 1967·Archives of Ophthalmology·D G Cogan, T Kuwabara
Jun 1, 1981·Analytical Biochemistry·P F KadorJ H Kinoshita
Jul 1, 1983·Microvascular Research·J D Gitlin, P A D'Amore

❮ Previous
Next ❯

Citations

Nov 1, 1991·Journal of Electron Microscopy Technique·R G Tilton
Jan 1, 1994·Documenta Ophthalmologica. Advances in Ophthalmology·J M van GervenJ A van Best
Dec 28, 1999·Brain Research. Brain Research Reviews·W E Thomas
Mar 18, 2003·Diabetes Research and Clinical Practice·Kazuma MiwaNigishi Hotta
Mar 21, 2000·Diabetes Research and Clinical Practice·Y L KaoM Silink
Jan 1, 1993·Journal of Ocular Pharmacology·Y TakahashiP F Kador
May 20, 2009·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Peter F KadorT Terasaki
Apr 1, 1992·Diabetic Medicine : a Journal of the British Diabetic Association·D K YueJ R Turtle
Jan 1, 1994·The British Journal of Ophthalmology·T A GardinerD B Archer
Feb 6, 2009·American Journal of Physiology. Cell Physiology·Elizabeth A ScheefNader Sheibani
Jul 21, 2012·Diabetes & Metabolic Syndrome·Dimitry A Chistiakov
Dec 15, 2007·Experimental Eye Research·Rūta Motiejūnaite, Andrius Kazlauskas
Dec 1, 2004·Diabetic Medicine : a Journal of the British Diabetic Association·K SiveniusM Uusitupa
Oct 19, 2007·Diabetes, Obesity & Metabolism·Sohail EjazChae Woong Lim
Jul 30, 2014·Diabetes/metabolism Research and Reviews·Touka BanaeeMehdi Farzadnia
Oct 22, 2013·Seminars in Ophthalmology·Ahmed F OmarJennifer K Sun
Jul 19, 2013·BioMed Research International·Giulia MalaguarneraTeresio Avitabile
Apr 23, 2016·Progress in Retinal and Eye Research·Peter F KadorPeter J Oates
Mar 20, 2001·Microvascular Research·P HelmboldI M Herman
Sep 28, 2002·Diabetes·Hans-Peter HammesUrban Deutsch
Feb 25, 2014·ISRN Ophthalmology·Joanna M TarrRakesh Chibber
May 5, 2017·The Journal of Clinical Endocrinology and Metabolism·Wen-Jun TuMin Guo
Oct 19, 2004·Seminars in Ophthalmology·F SimonelliF Bandello
Dec 12, 2020·Antioxidants·Thangal YumnamchaAhmed S Ibrahim
Jun 1, 1990·Experimental Eye Research·P F Kador

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.