Aldose reductase prevents aldehyde toxicity in cultured human lens epithelial cells

Experimental Eye Research
Agnieszka PladzykSatish K Srivastava

Abstract

Aldehydes are widespread environmental and industrial compounds, which cause cytotoxicity, tissue damage, mutagenicity, and carcinogenicity leading to various disease conditions such as cardiovascular, bronchial, and visual complications. We have shown earlier that aldose reductase (AR) besides reducing glucose to sorbitol, efficiently reduces various toxic lipid-derived aldehydes, generated under oxidative stress, with K(m) in the physiological range. We have identified the role of AR in the prevention of various lipid aldehyde-induced cytotoxic signals leading to apoptosis in human lens epithelial cells (HLEC). HLEC were cultured without or with AR inhibitors followed by addition of various saturated and unsaturated lipid aldehydes with a carbon chain length varying from C3 to C10. The cell viability was assessed by cell counts and MTT assay, and apoptosis was measured by evaluating nucleosomal degradation and caspase-3 activation using specific ELISA kits. Although all the aldehydes caused apoptosis of HLEC, the unsaturated aldehydes were more toxic than saturated aldehydes. Inhibition of AR by sorbinil potentiated while the over-expression of AR prevented the apoptosis induced by various lipid aldehydes. AR over-expression ...Continue Reading

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Citations

Dec 22, 2007·Toxicology and Applied Pharmacology·Umesh C S YadavSatish K Srivastava
Nov 23, 2006·Journal of the Peripheral Nervous System : JPNS·Yun JiangAndrew P Mizisin
Jun 12, 2014·Lasers in Surgery and Medicine·Janet AjdaharianPetra Wilder-Smith
Mar 9, 2021·Frontiers in Endocrinology·Josy AugustineTim M Curtis

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