ALG-2 participates in recovery of cells after plasma membrane damage by electroporation and digitonin treatment

PloS One
Jonas M la CourM W Berchtold

Abstract

The calcium binding protein ALG-2 is upregulated in several types of cancerous tissues and cancer cell death may be a consequence of ALG-2 downregulation. Novel research suggests that ALG-2 is involved in membrane repair mechanisms, in line with several published studies linking ALG-2 to processes of membrane remodeling and transport, which may contribute to the fitness of cells or protect them from damage. To investigate the involvement of ALG-2 in cell recovery after membrane damage we disrupted the PDCD6 gene encoding the ALG-2 protein in DT-40 cells and exposed them to electroporation. ALG-2 knock-out cells were more sensitive to electroporation as compared to wild type cells. This phenotype could be reversed by reestablishing ALG-2 expression confirming that ALG-2 plays an important role in cell recovery after plasma membrane damage. We found that overexpression of wild type ALG-2 but not a mutated form unable to bind Ca2+ partially protected HeLa cells from digitonin-induced cell death. Further, we were able to inhibit the cell protective function of ALG-2 after digitonin treatment by adding a peptide with the ALG-2 binding sequence of ALIX, which has been proposed to serve as the ALG-2 downstream target in a number of pr...Continue Reading

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Citations

Jan 29, 2021·International Journal of Molecular Sciences·Ryuta InukaiHideki Shibata
Jul 31, 2020·Current Opinion in Cell Biology·Kevin P Bohannon, Phyllis I Hanson
Jun 20, 2021·Bioelectrochemistry·Tina Batista NapotnikDamijan Miklavčič

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Methods Mentioned

BETA
PCR
transfection
gene knock-out

Software Mentioned

GraphPad Prism

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