Algal cell response to laboratory-induced cadmium stress: a multimethod approach

European Biophysics Journal : EBJ
Nadica Ivošević DeNardisBéla Gyurcsik

Abstract

We examined the response of algal cells to laboratory-induced cadmium stress in terms of physiological activity, autonomous features (motility and fluorescence), adhesion dynamics, nanomechanical properties, and protein expression by employing a multimethod approach. We develop a methodology based on the generalized mathematical model to predict free cadmium concentrations in culture. We used algal cells of Dunaliella tertiolecta, which are widespread in marine and freshwater systems, as a model organism. Cell adaptation to cadmium stress is manifested through cell shape deterioration, slower motility, and an increase of physiological activity. No significant change in growth dynamics showed how cells adapt to stress by increasing active surface area against toxic cadmium in the culture. It was accompanied by an increase in green fluorescence (most likely associated with cadmium vesicular transport and/or beta-carotene production), while no change was observed in the red endogenous fluorescence (associated with chlorophyll). To maintain the same rate of chlorophyll emission, the cell adaptation response was manifested through increased expression of the identified chlorophyll-binding protein(s) that are important for photosynth...Continue Reading

References

Feb 1, 1994·Archives of Environmental Contamination and Toxicology·I Visviki, J W Rachlin
Feb 24, 2001·Bioelectrochemistry·V SvetlicićV Zutić
Jun 11, 2002·Biochemical and Biophysical Research Communications·Naoki TsujiKazuhisa Miyamoto
Oct 3, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Peter G C CampbellBernard Vigneault
May 6, 2005·Nature·Todd W LaneFrançois M M Morel
Aug 17, 2005·Biomolecular Engineering·K NikookarL Hosseini
Dec 13, 2006·Aquatic Toxicology·Virginia García-RíosGerardo Gold-Bouchot
Oct 6, 2007·Biological Trace Element Research·Melek Türker SaçanSehnaz Bolkent
Dec 7, 2007·Pflügers Archiv : European journal of physiology·Mark HildebrandDavid P Allison
Nov 22, 2008·Journal of Hazardous Materials·S FolgarJ Abalde
Sep 15, 2009·Aquatic Toxicology·Meng-Jiao Wang, Wen-Xiong Wang
Apr 13, 2011·Journal of Plant Physiology·Sridevi DamarajuBernhard Grimm
Apr 14, 2012·Plant Signaling & Behavior·Lichuan Wan, Haiyan Zhang
Jul 7, 2012·Bioelectrochemistry·Nadica Ivošević DenardisPrimož Ziherl
Oct 17, 2015·International Journal of Molecular Sciences·Krystian MiazekDorothee Goffin
Nov 22, 2015·Cellular and Molecular Life Sciences : CMLS·Barbara MessnerDavid Bernhard
Jan 16, 2016·Sensors and Actuators. B, Chemical·Samuel S R DasaryAnant K Singh
Mar 4, 2019·Bioelectrochemistry·Flavien PilletNadica Ivošević DeNardis

❮ Previous
Next ❯

Citations

Apr 10, 2020·Bioelectrochemistry·Nadica Ivošević DeNardisP Thomas Vernier
Feb 20, 2020·Methods and Applications in Fluorescence·A Marcek ChorvatovaD Chorvat

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
optical microscopy
AFM
electrophoresis
Profiler
Fluorescence
fluorescence microscopy
confocal microscopy

Software Mentioned

ICY
R Project for Statistical Computing and Graphics
CLUSTAL O
Motion Profiler
R
JPKSPM
LabView
ZEN
SPCImage
Mascot

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.