Algal polysaccharides as matrices for the immobilization of urease in lipid ultrathin films studied with tensiometry and vibrational spectroscopy: Physical-chemical properties and implications in the enzyme activity

Colloids and Surfaces. B, Biointerfaces
Audrey Kalinouski de BritoLuciano Caseli

Abstract

Currently, many biological substances extracted from algae have received special attention because of their intrinsic characteristics, which can be applied to different areas of biotechnology. Therefore, in the current study, exopolysaccharides (EPS) from the microalgae Cryptomonas tetrapirenoidosa were employed as an aqueous subphase of a monolayer formed by the lipid dioctadecyldimethylammonium bromide (DODAB). The primary objective of this approach was to evaluate whether EPS could serve as a matrix for the immobilization of the enzyme urease to produce biosensors for urea. After DODAB was spread on the EPS solutions, urease was injected into the aqueous subphase, and the surface was submitted to compression using lateral barriers. The monolayers were subsequently characterized by surface pressure-area isotherms and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The results indicated that EPS enhanced the adsorption of the enzyme on the lipid monolayer. The mixed films were later transferred to solid supports using the Langmuir-Blodgett (LB) technique and were characterized by transfer ratio, PM-IRRAS, quartz crystal microbalance, and atomic force microscopy. The immobilization of the enzyme ...Continue Reading

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Citations

May 28, 2016·Journal of Colloid and Interface Science·Camila Gouveia BarbosaLaura Oliveira Péres
Apr 25, 2020·Colloids and Surfaces. B, Biointerfaces·Luiz Henrique Rodrigues Rola PossarleLuciano Caseli
Feb 6, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Raul T RodriguesLuciano Caseli

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