Alginate immobilization of recombinant Escherichia coli whole cells harboring L-arabinose isomerase for L-ribulose production

Bioprocess and Biosystems Engineering
Ye-Wang ZhangJung-Kul Lee

Abstract

Recombinant Escherichia coli whole cells harboring Bacillus licheniformis L-arabinose isomerase (BLAI) were immobilized with alginate. The operational conditions for immobilization were optimized with response surface methodology. Optimal alginate concentration, Ca(2+) concentration, and cell mass loading were 1.8% (w/v), 0.1 M, and 44.5 g L(-1), respectively. The interactions between Ca(2+) concentration, alginate concentration, and initial cell mass were significant. After immobilization of BLAI, cross-linking with 0.1% glutaraldehyde significantly reduced cell leakage. The half-life of immobilized whole cells was 150 days, which was 50-fold longer than that of free cells. In seven repeated batches for L-ribulose production, the productivity was as high as 56.7 g L(-1) h(-1) at 400 g L(-1) substrate concentration. The immobilized cells retained 89% of the initial yield after 33 days of reaction. Immobilization of whole cells harboring BLAI, therefore, makes a suitable biocatalyst for the production of L-ribulose, particularly because of its high stability and low cost.

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Citations

May 25, 2010·Applied Microbiology and Biotechnology·Ye-Wang ZhangJung-Kul Lee
Aug 26, 2014·Jundishapur Journal of Microbiology·Peyman Derikvand, Zahra Etemadifar
Oct 5, 2014·Applied Microbiology and Biotechnology·Zheng XuHong Xu
Nov 3, 2010·Applied Microbiology and Biotechnology·Ye-Wang ZhangJung-Kul Lee
Sep 28, 2015·Bioprocess and Biosystems Engineering·Lidija DjokicJasmina Nikodinovic-Runic
Feb 9, 2012·International Journal of Biological Macromolecules·Seçkin Gülay, Gülşah Şanlı-Mohamed
May 29, 2015·Biotechnology, Biotechnological Equipment·Nur Royhaila MohamadRoswanira Abdul Wahab
Mar 15, 2011·Carbohydrate Research·Geeta Meher, Ramanarayanan Krishnamurthy
Oct 8, 2016·Microbial Cell Factories·Mercedes Berlanga, Ricardo Guerrero
Nov 6, 2018·Critical Reviews in Biotechnology·Muhammad BilalHafiz M N Iqbal
May 7, 2020·Applied Microbiology and Biotechnology·Jiajun ChenWanmeng Mu
Jun 12, 2021·Food Research International·Shahid MahmoodWanmeng Mu
Aug 12, 2021·Brazilian Journal of Biology = Revista Brasleira De Biologia·Y HuangZ Zhao

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