Alginate microencapsulation of human islets does not increase susceptibility to acute hypoxia

Journal of Diabetes Research
I K HalsV Grill

Abstract

Islet transplantation in diabetes is hampered by the need of life-long immunosuppression. Encapsulation provides partial immunoprotection but could possibly limit oxygen supply, a factor that may enhance hypoxia-induced beta cell death in the early posttransplantation period. Here we tested susceptibility of alginate microencapsulated human islets to experimental hypoxia (0.1-0.3% O2 for 8 h, followed by reoxygenation) on viability and functional parameters. Hypoxia reduced viability as measured by MTT by 33.8 ± 3.5% in encapsulated and 42.9 ± 5.2% in nonencapsulated islets (P < 0.2). Nonencapsulated islets released 37.7% (median) more HMGB1 compared to encapsulated islets after hypoxic culture conditions (P < 0.001). Glucose-induced insulin release was marginally affected by hypoxia. Basal oxygen consumption was equally reduced in encapsulated and nonencapsulated islets, by 22.0 ± 6.1% versus 24.8 ± 5.7%. Among 27 tested cytokines/chemokines, hypoxia increased the secretion of IL-6 and IL-8/CXCL8 in both groups of islets, whereas an increase of MCP-1/CCL2 was seen only with nonencapsulated islets. Conclusion. Alginate microencapsulation of human islets does not increase susceptibility to acute hypoxia. This is a positive findi...Continue Reading

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Citations

May 28, 2015·Journal of Biomedical Materials Research. Part a·Kjetil FormoBerit L Strand
Apr 24, 2015·Journal of Biomedical Materials Research. Part a·Yukinori TsukudaNorimasa Iwasaki
Feb 6, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shuai XueHaifeng Ye
May 20, 2017·Current Diabetes Reports·Mustafa OmamiJosé Oberholzer
Apr 24, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Rui CaoJonathan R T Lakey
May 14, 2016·The International Journal of Risk & Safety in Medicine·Camila Dos ReisRui Santos Cruz
Feb 12, 2017·Stem Cells Translational Medicine·Berit L StrandGudmund Skjak-Braek
Jun 12, 2020·Journal of Tissue Engineering·Jordan MagissonSéverine Sigrist
Feb 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Virginia SpanoudakiDaniel G Anderson

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Methods Mentioned

BETA
ELISA

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