Alginate microparticles loaded with lipopolysaccharide subunit antigen for mucosal vaccination against Klebsiella pneumoniae

Biologicals : Journal of the International Association of Biological Standardization
R R JainM D Menon

Abstract

Klebsiella pneumoniae (K. pneumoniae) is one of the commonest causes of nosocomial infections in human beings. Since K. pneumoniae infections are air borne type, controlling it by mucosal vaccination through nasal and pulmonary route could be a promising approach in order to simulate the natural infection. New vaccines such as subunit vaccines are safer than traditional vaccines, but they are less immunogenic. Therefore to enhance their immunogenicity, there is a need to develop potent and safe adjuvants and delivery systems. It has been established that micro-particles are one of the most potent adjuvants available for mucosal delivery of vaccines and they do so by improving uptake of encapsulated antigen by antigen presenting cells (APCs). Lipopolysaccharide (LPS), the antigenic fraction was extracted from K. pneumoniae by hot phenol extraction method. LPS loaded sodium alginate microparticles were prepared by emulsion ionic gelation method. Microparticles with particle size less than 5 μm were obtained. Loading efficiency of the LPS loaded microparticles ranged from 76 to 82 %. Comparative in vivo immunogenicity studies were carried for free LPS and encapsulated LPS, administered via intramuscular, intratracheal and intranas...Continue Reading

References

Jan 1, 1977·Journal of Biological Standardization·K H WongE B Seligmann
Jul 1, 1992·Journal of Microencapsulation·L S WanL W Chan
Sep 1, 1969·The Biochemical Journal·A H Fensom, G W Gray
Apr 1, 1967·Analytical Biochemistry·J R Loewenberg
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jan 1, 1984·Annual Review of Medicine·J Bienenstock
Apr 3, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E LautenbachN O Fishman
Aug 1, 1959·Biochimica Et Biophysica Acta·L KLUNGSOYR
Jun 19, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David L PatersonVictor L Yu
Nov 23, 2006·International Journal of Pharmaceutics·R RastogiA K Mishra
Aug 30, 2008·Vaccine·Abigail ClementsRichard A Strugnell
Jan 11, 2011·Indian Journal of Pharmaceutical Sciences·J J ParmarMala D Menon

❮ Previous
Next ❯

Citations

Jun 30, 2019·Expert Review of Vaccines·Myeongjin ChoiAlan S Cross
Jul 5, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eva Torres-SangiaoMonica Cartelle Gestal
Oct 26, 2018·International Journal of Molecular Sciences·Ding-Ping BaiXi-Feng Zhang
Dec 2, 2020·Marine Drugs·Yuliya KhrunykHermann Ehrlich
Dec 9, 2020·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
May 7, 2021·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
Jul 20, 2021·Advanced Drug Delivery Reviews·Chao PanHeng-Liang Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alstrom Syndrome

Alstrom syndrome is a rare disorder characterized by early obesity, loss of central vision, diabetes mellitus, hearing loss, and short stature. Here is the latest research.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.