Abstract
Klebsiella pneumoniae (K. pneumoniae) is one of the commonest causes of nosocomial infections in human beings. Since K. pneumoniae infections are air borne type, controlling it by mucosal vaccination through nasal and pulmonary route could be a promising approach in order to simulate the natural infection. New vaccines such as subunit vaccines are safer than traditional vaccines, but they are less immunogenic. Therefore to enhance their immunogenicity, there is a need to develop potent and safe adjuvants and delivery systems. It has been established that micro-particles are one of the most potent adjuvants available for mucosal delivery of vaccines and they do so by improving uptake of encapsulated antigen by antigen presenting cells (APCs). Lipopolysaccharide (LPS), the antigenic fraction was extracted from K. pneumoniae by hot phenol extraction method. LPS loaded sodium alginate microparticles were prepared by emulsion ionic gelation method. Microparticles with particle size less than 5 μm were obtained. Loading efficiency of the LPS loaded microparticles ranged from 76 to 82 %. Comparative in vivo immunogenicity studies were carried for free LPS and encapsulated LPS, administered via intramuscular, intratracheal and intranas...Continue Reading
References
Jan 1, 1977·Journal of Biological Standardization·K H WongE B Seligmann
Jul 1, 1992·Journal of Microencapsulation·L S WanL W Chan
Dec 1, 1990·Canadian Journal of Microbiology·M RaniS Chhibber
Jul 1, 1988·Annales De L'Institut Pasteur. Immunology·J M PostalY Crenn
Dec 13, 1985·Science·A G HarmsenD E Bice
Sep 1, 1969·The Biochemical Journal·A H Fensom, G W Gray
Apr 1, 1967·Analytical Biochemistry·J R Loewenberg
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jan 1, 1984·Annual Review of Medicine·J Bienenstock
Oct 31, 1998·Vaccine·J E EylesH O Alpar
Apr 3, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E LautenbachN O Fishman
Aug 1, 1959·Biochimica Et Biophysica Acta·L KLUNGSOYR
Dec 17, 2003·Analytical Chemistry·Sofia GulinEugenio Hardy
Jun 19, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David L PatersonVictor L Yu
Jun 16, 2005·Folia Microbiologica·V YadavS Chhibber
Nov 23, 2006·International Journal of Pharmaceutics·R RastogiA K Mishra
Aug 30, 2008·Vaccine·Abigail ClementsRichard A Strugnell
Jan 11, 2011·Indian Journal of Pharmaceutical Sciences·J J ParmarMala D Menon
Feb 22, 2012·Drug Development and Industrial Pharmacy·Xin TangWei-san Pan
Citations
Oct 6, 2017·Frontiers in Microbiology·Litty BabuPrakash N Reddy
Apr 10, 2019·Polymers·Manuel J LisFabricio Maestá Bezerra
Jun 30, 2019·Expert Review of Vaccines·Myeongjin ChoiAlan S Cross
Jul 5, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eva Torres-SangiaoMonica Cartelle Gestal
Oct 26, 2018·International Journal of Molecular Sciences·Ding-Ping BaiXi-Feng Zhang
Dec 2, 2020·Marine Drugs·Yuliya KhrunykHermann Ehrlich
Dec 9, 2020·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
May 24, 2020·Vaccine·Marjory Xavier RodriguesRodrigo Carvalho Bicalho
May 7, 2021·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
May 26, 2021·Advanced Science·Zhehui PengLi Zhu
Jul 20, 2021·Advanced Drug Delivery Reviews·Chao PanHeng-Liang Wang