Alginate Oligosaccharide-Induced Modification of the lasI-lasR and rhlI-rhlR Quorum-Sensing Systems in Pseudomonas aeruginosa

Antimicrobial Agents and Chemotherapy
Alison A JackKatja E Hill

Abstract

Pseudomonas aeruginosa plays a major role in many chronic infections. Its ability to readily form biofilms contributes to its success as an opportunistic pathogen and its resistance/tolerance to antimicrobial/antibiotic therapy. A low-molecular-weight alginate oligomer (OligoG CF-5/20) derived from marine algae has previously been shown to impair motility in P. aeruginosa biofilms and disrupt pseudomonal biofilm assembly. As these bacterial phenotypes are regulated by quorum sensing (QS), we hypothesized that OligoG CF-5/20 may induce alterations in QS signaling in P. aeruginosa QS regulation was studied by using Chromobacterium violaceum CV026 biosensor assays that showed a significant reduction in acyl homoserine lactone (AHL) production following OligoG CF-5/20 treatment (≥2%; P < 0.05). This effect was confirmed by liquid chromatography-mass spectrometry analysis of C4-AHL and 3-oxo-C12-AHL production (≥2%; P < 0.05). Moreover, quantitative PCR showed that reduced expression of both the las and rhl systems was induced following 24 h of treatment with OligoG CF-5/20 (≥0.2%; P < 0.05). Circular dichroism spectroscopy indicated that these alterations were not due to steric interaction between the AHL and OligoG CF-5/20. Confoc...Continue Reading

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Citations

Jun 21, 2020·Nutrients·Mostafa AsadpoorSaskia Braber
Jun 6, 2020·Applied Microbiology and Biotechnology·Subendu Sarkar
Nov 4, 2020·Journal of Infection and Public Health·Nilufer Uzunbayir-AkelMine Hosgor-Limoncu
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Jun LiuZhengqiang Jiang
Nov 22, 2020·Marine Drugs·Anne TøndervikHåvard Sletta
Jun 3, 2021·International Journal of Molecular Sciences·Suqi HaoHuaqiao Tang
Jun 14, 2021·Carbohydrate Polymers·Ushasree Mrudulakumari VasudevanEun Yeol Lee
Oct 31, 2021·Applied Microbiology and Biotechnology·Rachith KalgudiTajalli Keshavarz

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