Abstract
Ever since proangiogenic growth factors have been used as a vascular medicine to treat tissue ischemia, efforts have been increasingly made to develop a method to enhance efficacy of growth factors in recreating microvascular networks, especially at low dose. To this end, we hypothesized that polysaccharides substituted with sulfate groups would amplify growth factor receptor activation and stimulate phenotypic activities of endothelial cells involved in neovascularization. We examined this hypothesis by modifying alginate with a controlled number of sulfates and using it to derive a complex with vascular endothelial growth factor (VEGF), as confirmed with fluorescence resonance energy transfer (FRET) assay. Compared with the bare VEGF and with a mixture of VEGF and unmodified alginates, the VEGF complexed with alginate sulfates significantly reduced the dissociation rate with the VEGFR-2, elevated VEGFR-2 phosphorylation level, and increased the number of endothelial sprouts in vitro. Furthermore, the VEGF-alginate sulfate complex improved recovery of perfusion in an ischemic hindlimb of a mouse due to the increase of the capillary density. Overall, this study not only demonstrates an important cofactor of VEGF but also uncove...Continue Reading
References
Dec 15, 1991·Journal of Immunological Methods·R KarlssonL Mattsson
Jan 23, 1999·Biomaterials·J A RowleyD J Mooney
Aug 25, 1999·Microvascular Research·C L GrosskreutzP A D'Amore
Dec 2, 1999·Nature Medicine·N Ferrara, K Alitalo
Aug 9, 2001·Artificial Organs·Y M ElçinG Gitnick
Jun 5, 2003·Nature Medicine·Rakesh K Jain
Jan 25, 2005·Cardiovascular Research·Tuomas TammelaKarri Paavonen
Jul 20, 2005·The Journal of Biological Chemistry·Satoko Ashikari-HadaKoji Kimata
Mar 7, 2006·Journal of the American College of Cardiology·Steven P Marso, William R Hiatt
Mar 31, 2006·Nature Reviews. Cancer·Raghu Kalluri, Michael Zeisberg
Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Anna-Karin OlssonLena Claesson-Welsh
Sep 14, 2006·Tissue Engineering·Matthias W LaschkeMichael D Menger
May 5, 2007·Thrombosis and Haemostasis·Nikolaos G Frangogiannis
Aug 7, 2007·Advanced Drug Delivery Reviews·Francesco Portaluppi, Björn Lemmer
Jan 10, 2008·Pharmaceutical Research·Hyun Joon KongDavid J Mooney
May 9, 2008·Biomaterials·Inbar FreemanSmadar Cohen
Aug 5, 2008·Bone·Zarana S PatelAntonios G Mikos
Nov 26, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Steven M Jay, W Mark Saltzman
Jun 2, 2009·Biomaterials·Lan CaoDavid J Mooney
Jun 3, 2009·Journal of Vascular Nursing : Official Publication of the Society for Peripheral Vascular Nursing·Rachelle L Muir
Nov 12, 2009·Cardiovascular Therapeutics·Antonia GermaniMaurizio C Capogrossi
Nov 13, 2009·Biomaterials·Eduardo A Silva, David J Mooney
Nov 3, 2010·Circulation·Gregorio BrevettiWilliam R Hiatt
May 28, 2011·Advanced Materials·Ross J DeVolderHyunjoon Kong
Citations
Jul 6, 2016·Molecular Pharmaceutics·Bumsoo Han
Nov 30, 2018·Journal of Biomedical Materials Research. Part a·Sajjad MohammadiMohsen Akbari
Jul 20, 2021·ACS Biomaterials Science & Engineering·Sung-Min KangShuichi Takayama