Alginate/Hydroxyapatite-Based Nanocomposite Scaffolds for Bone Tissue Engineering Improve Dental Pulp Biomineralization and Differentiation

Stem Cells International
Silvia SancilioAmelia Cataldi

Abstract

Tissue engineering is widely recognized as a promising approach for bone repair and reconstruction. Several attempts have been made to achieve materials that must be compatible, osteoconductive, and osteointegrative and have mechanical strength to provide a structural support. Composite scaffolds consisting in biodegradable natural polymers are very promising constructs. Hydroxyapatite (HAp) can support alginate as inorganic reinforcement and osteoconductive component of alginate/HAp composite scaffolds. Therefore, HAp-strengthened polymer biocomposites offer a solid system to engineer synthetic bone substitutes. In the present work, HAp was incorporated into an alginate solution and internal gelling was induced by addition of slowly acid-hydrolyzing D-gluconic acid delta-lactone for the direct release of calcium ions from HAp. It has been previously demonstrated that alginate-based composites efficiently support adhesion of cancer bone cell lines. Human dental pulp stem cells (DPSCs) identified in human dental pulp are clonogenic cells capable of differentiating in multiple lineage. Thus, this study is aimed at verifying the mineralization and differentiation potential of human DPSCs seeded onto scaffolds based on alginate and...Continue Reading

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Citations

Apr 4, 2019·Nanomaterials·Moumita GhoshLihi Adler-Abramovich
Apr 28, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Qiaoqiao JinZhengwei Huang
Jul 28, 2019·Materials·Naimah ZeinFlorence Fioretti
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May 18, 2020·International Journal of Molecular Sciences·Håvard Jostein HaugenJanne Elin Reseland
Apr 23, 2020·International Journal of Molecular Sciences·Viviana di GiacomoSilvia Sancilio
Jan 28, 2021·International Journal of Molecular Sciences·Giulia BattafaranoAndrea Del Fattore
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Aug 1, 2020·ACS Applied Bio Materials·Divya PankajakshanMarco C Bottino
Aug 25, 2021·Journal of Materials Science. Materials in Medicine·Soo-Ling BeeZ A Abdul Hamid

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Methods Mentioned

BETA
flow cytometry
Assay
ELISA
electrophoresis
PCR

Software Mentioned

QuantiOne
GraphPad Prism
GraphPad

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