Aligned Immobilization of Proteins Using AC Electric Fields

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Eva-Maria LauxR Hölzel

Abstract

Protein molecules are aligned and immobilized from solution by AC electric fields. In a single-step experiment, the enhanced green fluorescent proteins are immobilized on the surface as well as at the edges of planar nanoelectrodes. Alignment is found to follow the molecules' geometrical shape with their longitudinal axes parallel to the electric field. Simultaneous dielectrophoretic attraction and AC electroosmotic flow are identified as the dominant forces causing protein movement and alignment. Molecular orientation is determined by fluorescence microscopy based on polarized excitation of the proteins' chromophores. The chromophores' orientation with respect to the whole molecule supports X-ray crystal data.

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Citations

Mar 4, 2020·Analytical and Bioanalytical Chemistry·Eva-Maria LauxRalph Hölzel
Jun 15, 2019·Biosensors·Honeyeh Matbaechi EttehadChristian Wenger
May 21, 2020·Micromachines·Honeyeh Matbaechi EttehadChristian Wenger
Apr 22, 2020·Analytical and Bioanalytical Chemistry·Mark A Hayes
May 28, 2020·Micromachines·Ralph Hölzel, Ronald Pethig
May 28, 2021·The Journal of Physical Chemistry. B·Rodrigo Noriega

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