Aligned nanofibers of decellularized muscle ECM support myogenic activity in primary satellite cells in vitro

Biomedical Materials
Krishna H PatelKoyal Garg

Abstract

Volumetric muscle loss (VML) is a loss of over ∼10% of muscle mass that results in functional impairment. Although skeletal muscle possesses the ability to repair and regenerate itself following minor injuries, VML injuries are irrecoverable. Currently, there are no successful clinical therapies for the treatment of VML. Previous studies have treated VML defects with decellularized extracellular matrix (D-ECM) scaffolds derived from either pig urinary bladder or small intestinal submucosa. These therapies were unsuccessful due to the poor mechanical stability of D-ECM leading to quick degradation in vivo. To circumvent these issues, in this manuscript aligned nanofibers of D-ECM were created using electrospinning that mimicked native muscle architecture and provided topographical cues to primary satellite cells. Additionally, combining D-ECM with polycaprolactone (PCL) improved the tensile mechanical properties of the electrospun scaffold. In vitro testing shows that the electrospun scaffold with aligned nanofibers of PCL and D-ECM supports satellite cell growth, myogenic protein expression, and myokine production.

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Citations

Feb 14, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Krishna H PatelKoyal Garg
Jun 23, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·John A TerrellChengpeng Chen
Aug 2, 2019·BioResearch Open Access·Anjali PatelKoyal Garg
Aug 23, 2020·Bioengineering·Mahdis Shayan, Ngan F Huang
Sep 13, 2020·Nanomaterials·Sara PolitiLaura Teodori
Oct 4, 2020·Bioengineering·Gregory ReidMichela Pozzobon
Apr 4, 2021·International Journal of Molecular Sciences·Natalia ZiemkiewiczKoyal Garg
Apr 27, 2021·Biomaterials Science·Ivan M BasurtoSteven R Caliari
Nov 24, 2020·Nanomedicine : Nanotechnology, Biology, and Medicine·Aditi JainKaushik Chatterjee
Aug 4, 2021·Essays in Biochemistry·Anita QuigleyRobert M I Kapsa
Aug 25, 2021·Regenerative Medicine·Kirthanashri S VasanthanDeepti Pandita
Jul 10, 2020·ACS Applied Materials & Interfaces·Allison N Ramey-WardKhalid Salaita
Sep 18, 2020·ACS Applied Materials & Interfaces·Seungju KimSandra E Kentish
Nov 18, 2021·Advanced Healthcare Materials·Liszt Y C Madruga, Matt J Kipper
Aug 20, 2021·Tissue Engineering. Part a·Ivan M BasurtoSteven R Caliari

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