Alignment and cell-matrix interactions of human corneal endothelial cells on nanostructured collagen type I matrices

Investigative Ophthalmology & Visual Science
Rita GruschwitzKatrin Engelmann

Abstract

To use nanoscopically defined, two-dimensional matrices assembled from aligned collagen type I fibrils as a sheet substratum for in vitro cultivation of human corneal endothelial cells (HCECs). To assess the effect of matrix architecture on HCEC morphology and to characterize integrin-mediated HCEC-matrix interaction. Cell alignment and cell-matrix interactions of primary HCECs and three different immortalized HCEC populations on native and UV-cross-linked collagen type I matrices were examined by time-lapse microscopy. Specific integrin α(2)β(1) binding to the collagen matrix was demonstrated using a function-blocking α(2) antibody. Integrin α(2) subunit expression levels of the four HCEC populations were analyzed by Western blot analysis. All HCEC populations aligned along the oriented collagen fibrils. Primary HCECs and, to a lesser extent, the other tested HCEC populations exerted high traction forces, leading to progressive matrix destruction. Cross-linking of the collagen matrices considerably increased matrix stability. Integrin subunit α(2) expression levels of the four cell types correlated with the degree of cell alignment and exertion of traction forces. In turn, blocking integrin subunit α(2) reduced cell alignment ...Continue Reading

Citations

Dec 4, 2014·Biophysical Journal·Kristin M RichingPatricia J Keely
Sep 11, 2016·Drug Delivery and Translational Research·Seppo RönkköArto Urtti
Sep 19, 2016·Experimental Eye Research·Maryam AliSara M Thomasy
Nov 5, 2016·Experimental Eye Research·Diana Santander-GarcíaJaime Millán
Jul 7, 2018·Beilstein Journal of Nanotechnology·Reika MakitaTsutomu Sugaya
Nov 3, 2020·Biofabrication·Naiana SuterDorothea Brüggemann
Jul 4, 2021·Experimental Eye Research·Meng-Chen Tsai, Julie T Daniels
Aug 13, 2018·ACS Biomaterials Science & Engineering·Michael G McCoyClaudia Fischbach

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