Alignment maps of tissues: I. Microscopic elliptical polarimetry

Biophysical Journal
Theodore T Tower, Robert T Tranquillo

Abstract

An automated method for generating a fiber alignment map in tissues, tissue-equivalents, and other fibrillar materials exhibiting linear and circular optical properties and scattering is presented. This method consists of interrogating the sample with elliptically polarized light from a rotated quarter-wave plate and an effective circular analyzer, and implementing nonlinear regression techniques to estimate parameters defining the optical properties of the optic train and the sample. Thus, an account is made for imperfect and misaligned optic elements. The optic train was modeled using the Mueller matrix representation and the combined sample properties by an exponential matrix. Because a sample's Mueller matrix does not uniquely determine the linear, circular, or scattering properties, the circular properties and effective scattering are estimated for a matched isotropic sample to determine and correct for the linear birefringence of an aligned sample. The method's utility is demonstrated by generating an alignment map of an arterial media-equivalent, a relevant test case because of its circumferential alignment and thus showing the method's sample orientation independence.

References

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Oct 19, 2001·Biophysical Journal·T T Tower, R T Tranquillo

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Citations

Apr 19, 2006·Annals of Biomedical Engineering·Ramji T VenkatasubramanianJohn C Bischof
Jul 17, 2009·Journal of Biomechanical Engineering·Choon-Sik JhunRobert T Tranquillo
Dec 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lee-Way JinBart Kahr
Oct 13, 2009·Journal of Biomechanics·Frank BaaijensNiels Driessen
May 22, 2009·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·Edward A SanderVictor H Barocas
Oct 19, 2001·Biophysical Journal·T T Tower, R T Tranquillo
Jul 31, 2012·Journal of Biomechanics·Derek J Lee, Beth A Winkelstein
Jul 9, 2004·Biophysical Journal·Oksana Kostyuk, Robert A Brown
Feb 26, 2005·Biophysical Journal·Olga K Dudko, George H Weiss
Jul 31, 2007·Microscopy Research and Technique·Luiza LarsenHubertus Axer
Jun 20, 2017·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·William J KowalskiBradley B Keller
Oct 11, 2008·Journal of Applied Physiology·Kyle P Quinn, Beth A Winkelstein
Jun 10, 2015·Annals of Biomedical Engineering·Amy A ClaesonVictor H Barocas

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