Alignment of astrocytes increases neuronal growth in three-dimensional collagen gels and is maintained following plastic compression to form a spinal cord repair conduit.

Tissue Engineering. Part a
Emma EastJames B Phillips

Abstract

After injury to the spinal cord, reactive astrocytes form a glial scar consisting of highly ramified cell processes that constitute a major impediment to repair, partly due to their lack of orientation and guidance for regenerating axons. In some nonmammalian vertebrates, successful central nervous system regeneration is attributed to the alignment of reactive glia, which guide axons across the lesion site. Here, a three-dimensional mammalian cell-seeded collagen gel culture system was used to explore the effect of astrocyte alignment on neuronal growth. Astrocyte alignment was mapped within tethered rectangular gels and was significantly greater at the edge and middle of the gels compared to the control unaligned regions. When neurons were seeded on and within astrocyte gels, neurite length was greatest in the areas of astrocyte alignment. There was no difference in expression of astrocyte reactivity markers between aligned and control areas. Having established the potential utility of astrocyte alignment, the aligned gels were plastic compressed, transforming them into mechanically robust implantable devices. After compression, astrocytes remained viable and aligned and supported neurite outgrowth, yielding a novel method for...Continue Reading

References

Oct 1, 1989·Developmental Biology·J W FawcettR L Meyer
Sep 1, 1988·The Journal of Cell Biology·K M NeugebauerL F Reichardt
Apr 1, 1981·Journal of Neuroscience Methods·G R DuttonK Tear
May 30, 1998·Cell Motility and the Cytoskeleton·M EastwoodR A Brown
Aug 6, 1999·Molecular and Cellular Neurosciences·M J WilbyJ W Fawcett
Sep 14, 1999·Brain Research Bulletin·J W Fawcett, R A Asher
Nov 10, 2001·Science·D H MauchF W Pfrieger
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·C P HofstetterL Olson
Apr 2, 2002·Experimental Neurology·Herbert M Geller, James W Fawcett
Oct 16, 2003·Spine·Satoru YoshiiAtaru Taniguchi
Nov 26, 2003·Experimental Neurology·Roy BiranPatrick A Tresco
Jan 22, 2004·Nature Reviews. Neuroscience·Jerry Silver, Jared H Miller
Feb 14, 2004·Biomaterials·J B Jennifer B RecknorS K Surya K Mallapragada
Feb 15, 2005·Cell·Karen S ChristophersonBen A Barres
Jun 21, 2005·Annals of the New York Academy of Sciences·Jennifer B RecknorS K Mallapragada
Nov 2, 2005·Tissue Engineering·James B PhillipsRobert A Brown
Dec 13, 2005·Annals of Biomedical Engineering·John A Pedersen, Melody A Swartz
Apr 29, 2006·Journal of Biology·Jeannette E DaviesStephen J A Davies
Jul 22, 2006·Nature Reviews. Neuroscience·Glenn Yiu, Zhigang He
Aug 31, 2006·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Betty P LiuStephen M Strittmatter
Jul 17, 2007·International Review of Cytology·Robert A Brown, James B Phillips
Aug 7, 2007·Neuron·Patrick Emery, Marc R Freeman
Aug 9, 2007·Nature Reviews. Molecular Cell Biology·Francesco PampaloniErnst H K Stelzer
Sep 1, 2007·Biomaterials·Charles James KirkpatrickRonald E Unger
Oct 2, 2007·Biomaterials·Annette SørensenSusan C Barnett
May 6, 2008·Tissue Engineering. Part B, Reviews·Jungwoo LeeNicholas A Kotov
May 7, 2008·Neuron Glia Biology·John K AlexanderRaymond J Colello
Jul 9, 2008·Biomaterials·Babette LanferCarsten Werner
May 14, 2009·Tissue Engineering. Part B, Reviews·Leann L NormanHelim Aranda-Espinoza
Sep 4, 2009·Journal of Neurology·Takeshi Nishio
Sep 29, 2009·Medical Molecular Morphology·Takahiro YaraToshihiko Taguchi
Oct 9, 2009·Journal of Tissue Engineering and Regenerative Medicine·Emma EastJames B Phillips
Oct 24, 2009·Molecular and Cellular Neurosciences·Ethan G HughesRita J Balice-Gordon

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Citations

Jan 10, 2012·Tissue Engineering. Part a·Ting LiuSing Yian Chew
Jun 19, 2012·Tissue Engineering. Part a·D Kacy CullenDouglas H Smith
Sep 11, 2012·Advanced Drug Delivery Reviews·Ensanya A Abou NeelJung Keun Hyun
Nov 5, 2014·Neural Regeneration Research·Christian B RicksRamesh Grandhi
Apr 2, 2014·Tissue Engineering. Part a·Tijna AlekseevaJames C Kirkpatrick
Dec 20, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wendy MartensIvo Lambrichts
Jan 31, 2016·Experimental Neurology·Hassan Al-AliVance P Lemmon
Mar 27, 2015·Journal of Functional Biomaterials·Hannah J LevisJulie T Daniels
Jan 5, 2016·Current Eye Research·Hannah J Levis, Julie T Daniels
Dec 25, 2012·Journal of the Mechanical Behavior of Biomedical Materials·Josephine P F WongRobert A Brown
Jan 15, 2015·ACS Applied Materials & Interfaces·Jae Myung JangNoo Li Jeon
Aug 16, 2011·Acta Biomaterialia·Mischa ZelzerNoah A Russell
Sep 29, 2015·Tissue Engineering. Part a·Laura A StruzynaD Kacy Cullen
Apr 3, 2012·Journal of Tissue Engineering and Regenerative Medicine·N ForrazC P McGuckin
Mar 5, 2014·Journal of Neurochemistry·Chew L LauPhilip M Beart
Apr 19, 2013·Journal of Biomedical Materials Research. Part a·Seul Ki MinHwa Sung Shin
Feb 7, 2015·Tissue Engineering. Part C, Methods·Christophe ChaubarouxJoseph Hemmerlé
Mar 1, 2012·Neuroscience Letters·Zin Z Khaing, Christine E Schmidt
Jan 7, 2014·Organogenesis·James B Phillips
Jul 10, 2013·Biomaterials·Melanie GeorgiouJames B Phillips
Dec 3, 2014·Progress in Neurobiology·Amy M HopkinsDavid L Kaplan
Jun 9, 2016·Journal of Tissue Engineering and Regenerative Medicine·Kritika S KatiyarD Kacy Cullen
Jun 23, 2016·Experimental Neurology·Jerry Silver
Jun 23, 2016·Advanced Healthcare Materials·Hironobu TakahashiTeruo Okano
Sep 19, 2016·Autonomic Neuroscience : Basic & Clinical·G F MartínezM M Brauer
Jan 5, 2017·Journal of Tissue Engineering and Regenerative Medicine·Kathleen SanenJames Phillips
Apr 6, 2017·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·P Eberwein, T Reinhard
Oct 25, 2013·Cell Transplantation·Zhihai FanHuanxiang Zhang
Feb 22, 2018·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Seon-Ah ChongIsabelle Niespodziany
Dec 1, 2017·Biomedical Materials·Russell Thompson, Shelly Sakiyama-Elbert
Oct 20, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Melissa L D RaynerJames B Phillips
Apr 22, 2014·Interface Focus·Mark Ahearne
Jan 21, 2020·Advanced Healthcare Materials·Beom Jin KimInsung S Choi

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

Analysis ® Pro
Improvision
Openlab

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