Alignment of gold clusters on DNA via a DNA-recognizing zinc finger-metallothionein fusion protein

Bioconjugate Chemistry
Shinya AriyasuTakeshi Yamamura

Abstract

The complementary recognition of base pairs (bp) is the major strategy in the "DNA lithography" of gold (Au)clusters or nanoparticles, where single-stranded DNAs sulfurized at their termini are generally used to bind Au clusters or nanoparticles. In this report, we discuss a new material that can be used to locate Au clusters on the desired positions of DNA. For this purpose, we combined a two-domain zinc finger (ZF) and the analogue of R domain of rat's liver metallothionein (MT) to utilize the DNA-recognizing ability of ZF motifs and the heavy metal binding ability of MTs, and prepared an artificial fusion protein, ZFZF-MTalpha (1). Titration experiments monitored by absorption and circular dichroism spectroscopies, as well as an electrophoretic mobility shift assay and quantification using 5,5'-dithiobis(2-nitrobenzoic acid), clarified that (1) the ZF domain traps two divalent metal ions to fold in a ZF structure with M(Cys)2(His)2 (M = Co, Zn, and Cd) coordination units, (2) the MT domain traps metal ions to form clusters (Cd2+ particularly forms a Cd4(Cys)9 cluster) without interfering with the folding of the ZF domain, and (3) 1 recognizes the 5'-GGGGGG-3' (G6) bp sequence in the presence of Zn2+ based on the amino acid s...Continue Reading

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May 7, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Shinya AriyasuTakeshi Yamamura

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Citations

Apr 12, 2011·Chemistry, an Asian Journal·Takeshi YamamuraRyota Sakamoto
Nov 28, 2013·Oncotarget·Akinori MoritaYoshio Hosoi
Apr 1, 2011·Journal of Inorganic Biochemistry·Yohei SanoTakashi Hayashi

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