Alignment of the cell nucleus from labeled proteins only for 4D in vivo imaging

Microscopy Research and Technique
B RiegerL J Van Vliet

Abstract

Studies of protein dynamics by 4D (3D + time) confocal microscopy in vivo are hampered by global cell motion. The time between the acquisitions of the 3D images is in the order of minutes. Therefore, it is not to be expected that the cell as a whole remains fixed in the water basin on the stage. This superimposes a motion on the protein dynamics that has to be removed. We present a robust registration technique to align the cell images that does not require the a priori establishment of point-to-point correspondences. Instead, it uses the distribution of the labeled proteins. After correction for the translation, the 3D rotation of the cell is estimated. A robust intrinsic body coordinate system is constructed via the inertia tensor from the intensity distribution. By combining basis transformation to this intrinsic coordinate system, we can calculated the rotation matrix in a conceptual and computational straightforward manner. We have evaluated the performance of this approach in three experiments with human osteaosarcoma cells (U-2 OS), where the nuclear proteins Histon H4 and PML were visualized. The PML is concentrated in several dozen nuclear spots. Expression of Histon H4 results in a total nuclear staining. The registra...Continue Reading

References

Feb 21, 1997·European Radiology·M Prokop, C M Schaefer-Prokop
Jan 29, 2000·Critical Reviews in Eukaryotic Gene Expression·R W DirksS P Snaar
Dec 6, 2003·The EMBO Journal·Chris MolenaarRoeland W Dirks
Feb 5, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Bernd Rieger, Lucas J van Vliet
Jan 1, 1996·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·B S Reddy, B N Chatterji

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Citations

Apr 15, 2005·Histochemistry and Cell Biology·Sabine M GörischKarsten Rippe
Mar 1, 2012·PloS One·Shan-e-Ahmed RazaNasir M Rajpoot
Jul 22, 2008·Journal of Microscopy·E GladilinK Rohr
Apr 9, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·S YangK Rohr
Sep 16, 2010·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Il-Han KimKarl Rohr
Aug 30, 2008·Biochimica Et Biophysica Acta·Malte WachsmuthKarsten Rippe
Jan 17, 2017·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Marco Tektonidis, Karl Rohr
Jun 3, 2010·Cold Spring Harbor Protocols·Karl RohrRoland Eils

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