Alix protein is substrate of Ozz-E3 ligase and modulates actin remodeling in skeletal muscle.

The Journal of Biological Chemistry
Antonella BongiovanniAlessandra D'Azzo

Abstract

Alix/AIP1 is a multifunctional adaptor protein that participates in basic cellular processes, including membrane trafficking and actin cytoskeleton assembly, by binding selectively to a variety of partner proteins. However, the mechanisms regulating Alix turnover, subcellular distribution, and function in muscle cells are unknown. We now report that Alix is expressed in skeletal muscle throughout myogenic differentiation. In myotubes, a specific pool of Alix colocalizes with Ozz, the substrate-binding component of the muscle-specific ubiquitin ligase complex Ozz-E3. We found that interaction of the two endogenous proteins in the differentiated muscle fibers changes Alix conformation and promotes its ubiquitination. This in turn regulates the levels of the protein in specific subcompartments, in particular the one containing the actin polymerization factor cortactin. In Ozz(-/-) myotubes, the levels of filamentous (F)-actin is perturbed, and Alix accumulates in large puncta positive for cortactin. In line with this observation, we show that the knockdown of Alix expression in C2C12 muscle cells affects the amount and distribution of F-actin, which consequently leads to changes in cell morphology, impaired formation of sarcolemma...Continue Reading

References

Jan 1, 1978·International Review of Cytology·F Grinnell
Jan 1, 1993·Annual Review of Cell Biology·J Condeelis
Feb 1, 1997·Current Opinion in Cell Biology·M D WelchT J Mitchison
Mar 24, 1999·The Journal of Cell Biology·C D Nobes, A Hall
Oct 19, 2001·Oncogene·S A Weed, J T Parsons
Dec 4, 2002·Nature Reviews. Molecular Cell Biology·David J KatzmannScott D Emr
Aug 2, 2003·Current Opinion in Cell Biology·Camilla RaiborgHarald Stenmark
Sep 25, 2003·Cell·Bettina StrackHeinrich G Göttlinger
Oct 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Juan Martin-SerranoAnton Yaravoy
Jan 7, 2004·Mechanisms of Development·Kálmán Somogyi, Pernille Rørth
Feb 13, 2004·Developmental Cell·Tommaso NastasiAlessandra D'Azzo
Oct 1, 2004·Molecular and Cellular Biology·Mirko H H SchmidtOliver Bögler
May 26, 2005·Journal of Cell Science·Alicia CabezasHarald Stenmark
Jun 7, 2005·Developmental Cell·Jaewon KimJames H Hurley
May 26, 2006·Nature Reviews. Molecular Cell Biology·Marko KaksonenDavid G Drubin
Jul 27, 2006·Journal of Cell Science·Greg Odorizzi
Sep 13, 2006·The Journal of Biological Chemistry·Shujuan PanJian Kuang
Apr 21, 2007·The EMBO Journal·Caleb RichterGreg Odorizzi
Jun 9, 2007·Science·Jez G Carlton, Juan Martin-Serrano
Jun 21, 2007·Current Biology : CB·Giles O C Cory, Peter J Cullen
Feb 5, 2008·In Vitro Cellular & Developmental Biology. Animal·Michele L MarquetteMarguerite Sognier
Jun 16, 2009·Structure·Ricardo PiresWinfried Weissenhorn
Sep 2, 2009·Proteins·Predrag RadivojacLilia M Iakoucheva
Jul 1, 2011·Journal of Virology·Qianting ZhaiWesley I Sundquist

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Citations

Nov 4, 2015·Annual Review of Physiology·Mamta Rai, Fabio Demontis
Mar 26, 2013·FEBS Letters·Daniele P RomancinoAntonella Bongiovanni
Apr 1, 2014·Current Opinion in Cell Biology·Michael R Dores, JoAnn Trejo
Nov 5, 2016·Cellular Signalling·Sili Liu, Gabrielle L Boulianne
Jun 8, 2019·ELife·David O AjasinVinayaka R Prasad
Jan 11, 2013·Development, Growth & Differentiation·Daniele P RomancinoMaria Di Bernardo
Aug 22, 2015·Development·Jessica WilliamsAaron N Johnson
Sep 20, 2017·Scientific Reports·Rosanna SalviaPatrizia Falabella
Oct 30, 2020·International Journal of Molecular Sciences·Jordan BlondelleStephan Lange
Sep 21, 2013·Journal of Cell Science·Sang-Min JangSangmyung Rhee

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