ALK translocation detection in non-small cell lung cancer cytological samples obtained by TBNA or EBUS-TBNA

Cytopathology : Official Journal of the British Society for Clinical Cytology
S BravacciniM Puccetti

Abstract

As the diagnosis of non-small cell lung cancer (NSCLC) is based on cytology in around 70% of cases, it is important to use the same material for molecular analyses. Fluorescence in situ hybridization (FISH) is the only approved test for the detection of the translocation and inversion of anaplastic lymphoma kinase (ALK), but the optimal procedures for the fixation or staining of the sample before FISH evaluation have not been established. We investigated whether ALK gene status determined by FISH in a prospectively enrolled case series of patients was affected by fixation and staining. One hundred and fifteen cytological samples were obtained by transbronchial needle aspiration (TBNA) or endobronchial ultrasound (EBUS)-TBNA from 109 patients with NSCLC. All samples were evaluated for epidermal growth factor receptor (EGFR) mutation by pyrosequencing and for ALK rearrangement by FISH. Specimens for ALK determination had been fixed with Cytofix(®) and/or Carnoy's solution or 10% formalin (cell blocks) and variously stained. Sixteen (14%) of the 115 samples were mutated for EGFR and 99 (86%) showed wild-type EGFR status. Of these 115 samples, 79 (69%) were negative for echinoderm microtubule-associated protein like 4 (EML4)-ALK tr...Continue Reading

References

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Jul 4, 2012·The European Respiratory Journal·Paola UliviMaurizio Puccetti
Feb 15, 2013·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Antonio MarchettiCarmine Pinto
Mar 28, 2013·Journal of Clinical Pathology·Paola UliviPiero Candoli

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Citations

Jul 26, 2017·Cancer Cytopathology·Pasquale PisapiaGiancarlo Troncone
Aug 29, 2018·Cytopathology : Official Journal of the British Society for Clinical Cytology·Aruna Nambirajan, Deepali Jain
Jan 29, 2019·Applied Immunohistochemistry & Molecular Morphology : AIMM·Xiaoyan WangLiang Cheng

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