PMID: 1194085Sep 7, 1975Paper

Alkali burns of the rabbit cornea. I. A histochemical study of beta-glucuronidase, beta-galactosidase and N-acetyl-beta-D-glucosaminidase

Histochemistry
J CejkováE Havránková

Abstract

In alkali burned rabbit corneas activities of beta-glucuronidase, N-acetyl-beta-D-glucosaminidase and acid beta-galactosidase were studied histochemically in various time intervals after the traumatization. The technic with semipermeable membranes was employed. Within four days after the injury enzyme activities in the traumatized area were almost lacking. The corresponding activities in the unaffected part of the cornea were within the norm. On the 7th day enzyme activities were on an increase (but still subnormal) in the traumatized area. This area was surrounded by a zone of keratocytes with high levels of enzyme activities. This was particularly remarkable in keratocytes subjacent to the epithelium. The activation of all enzymes studied was present in the basal layer of the epithelium and in the endothelium as well. On the 14th day enzyme activities in the traumatized area were nearly restored and on the 32nd day they could not be distinguished from the normal cornea. Beta-galactosidase displayed a relatively maximal increase in the activity of all enzymes investigated.

References

Jul 1, 1969·Archives of Ophthalmology·H H SlanskyC H Dohlman
Aug 1, 1974·Archives of Ophthalmology·M A Lemp
Dec 31, 1973·Histochemie. Histochemistry. Histochimie·Z Lojda
Mar 1, 1969·Archives of Ophthalmology·S I BrownH E Wassermann

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Citations

Jan 1, 1989·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·M ZierhutU Pleyer
Jan 1, 1986·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·S HaraK Mizuno
Jan 1, 1985·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·T Pahlitzsch, P Sinha
Nov 30, 1979·Documenta Ophthalmologica. Advances in Ophthalmology·J François, V Victoria-Troncoso
Jan 1, 1976·Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie. Albrecht Von Graefe's Archive for Clinical and Experimental Ophthalmology·A Bolková, J Obenberger
Jan 1, 1983·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·A Bolková, J Cejková
Jan 1, 1986·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·T ShionoK Mizuno
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·S R Gordon

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