PMID: 4964763Oct 1, 1967

Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase

The Biochemical Journal
R P CoxM J Griffin

Abstract

Alkaline phosphatase prepared from mammalian cell cultures was found to have alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by: (1) concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; (2) proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activities by titrating constant amounts of an enzyme preparation with increasing concentration of antibody; (3) immune electrophoresis, which showed that precipitin bands that have alkaline phosphatase activity also have pyrophosphatase activity; (4) inhibition of pyrophosphatase activity by cysteine, an inhibitor of alkaline phosphatase activity; (5) similar subcellular localization of the two enzyme activities as demonstrated by histochemical methods; (6) hormonal and substrate induction of alkaline phosphatase activity in mammalian cell cultures, which produced a nearly parallel rise in inorganic pyrophosphatase activity.

Citations

Mar 15, 2015·Stem Cells International·Kateřina ŠtefkováJiří Pacherník
Nov 25, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·K B RajaT J Peters
Oct 15, 1975·Experimental Cell Research·I Singh, K Y Tsang
Jan 1, 1974·Annals of Human Genetics·R A FisherH Harris
Nov 4, 2009·The Journal of Rheumatology·Jovil V A KannampuzhaKenneth P H Pritzker
Oct 14, 1969·Annals of the New York Academy of Sciences·N K Ghosh
Aug 15, 1969·Annals of the New York Academy of Sciences·W E Wacker
Jan 1, 1982·Acta Odontologica Scandinavica·J Friskopp, L Hammarström
May 1, 1978·Arthritis and Rheumatism·S JacobelliM Sapag-Hagar

Related Concepts

Alkaline Phosphatase
Anthropoidea
Enzyme Induction
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Immunoelectrophoresis
Kidney
Orthophosphate
Precipitin Tests
Predonine
Pyrophosphatase

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