Abstract
A third plasmid-mediated colistin resistance gene, mcr-3, is increasingly being reported in Enterobacteriaceae and Aeromonas spp. from animals and humans. To investigate the molecular epidemiology of mcr in the gut flora of Chinese outpatients, 152 stool specimens were randomly collected from outpatients in our hospital from May to June, 2017. Stool specimens enriched in alkaline peptone water or Luria-Bertani (LB) broth were screened for mcr-1, mcr-2, and mcr-3 using polymerase chain reaction (PCR)-based assays. Overall, 19.1% (29/152) and 5.3% (8/152) of the stool samples enriched in alkaline peptone water were PCR-positive for mcr-1 and mcr-3, respectively, while 2.7% (4/152) of samples were positive for both mcr-1 and mcr-3. Strains isolated from the samples that were both mcr-1- and mcr-3-positive were subjected to antimicrobial susceptibility testing by broth microdilution. They were also screened for the presence of other resistance genes by PCR, while multilocus sequence typing and whole-genome sequencing were used to investigate the molecular epidemiology and genetic environment, respectively, of the resistance genes. mcr-3-positive Aeromonas veronii strain 126-14, containing a mcr-3.8-mcr-3-like2 segment, and mcr-1-po...Continue Reading
References
Mar 15, 2006·The Journal of Infection·Yunsong YuYilin Ma
Jun 16, 2012·TheScientificWorldJournal·Isoken H IgbinosaAnthony I Okoh
Oct 23, 2013·Antimicrobial Agents and Chemotherapy·Sushim Kumar GuptaJean-Marc Rolain
Nov 26, 2014·Genome Medicine·Michael InouyeKathryn E Holt
May 7, 2015·Foodborne Pathogens and Disease·Tanuja K G M GowdaArchana R Patil
Nov 26, 2015·The Lancet Infectious Diseases·Yi-Yun LiuJianzhong Shen
Jul 16, 2016·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Basil Britto XavierSurbhi Malhotra-Kumar
Jul 19, 2016·Journal of Fish Diseases·H Y Chenia, S Duma
Mar 1, 2017·Antimicrobial Agents and Chemotherapy·Yong-Qiang YangHong-Ning Wang
Jun 1, 2017·Antimicrobial Agents and Chemotherapy·Hong-Wei ZhouGong-Xiang Chen
Jun 29, 2017·MBio·Wenjuan YinYang Wang
Jul 3, 2017·International Journal of Antimicrobial Agents·Yan-Yan HuGong-Xiang Chen
Jul 7, 2017·3 Biotech·Gunajit GoswamiMadhumita Barooah
Aug 12, 2017·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Eva LitrupMia Torpdahl
Aug 12, 2017·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Marta HernándezAlberto Quesada
Aug 20, 2017·BMC Microbiology·Lívia Christina Alves da SilvaNilma Cintra Leal
Aug 30, 2017·Antimicrobial Agents and Chemotherapy·Zhuoren LingJianzhong Shen
Oct 4, 2017·Antimicrobial Agents and Chemotherapy·Lu LiuZhiyong Zong
Oct 19, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Lan-Lan ZhongGuo-Bao Tian
Citations
Dec 5, 2019·Antimicrobial Resistance and Infection Control·Vu Thi Ngoc BichHeiman F L Wertheim
Nov 30, 2020·Journal of Global Antimicrobial Resistance·Yu ZengRong Zhang
Jun 15, 2021·Archives of Microbiology·Shuangfang HuYuebin Ke
Jul 1, 2021·Antimicrobial Resistance and Infection Control·Yolandi SnymanMae Newton-Foot