Alkaline Phosphatase Activity of Staphylococcus aureus Grown in Biofilm and Suspension Cultures

Current Microbiology
Kevin M Danikowski, Tong Cheng

Abstract

Staphylococcus aureus is known for its resistance to antibiotic treatment as well as the ability to form biofilms. Biofilm formation has been seen in S. aureus infections, yet, the mechanism of biofilm formation is not completely understood. Many molecules, such as DNA and polysaccharides, have been identified in the biofilm microenvironment, but little is known about the enzymes involved in the process. In this paper, alkaline phosphatase (ALP) activity was investigated in S. aureus grown either in biofilm or suspension cultures, achieved using DNase I. A significant increase of ALP activity was observed in S. aureus biofilm culture compared to its suspension counterpart. Treatment of sodium orthovanadate, an ALP inhibitor, significantly decreased biofilm formation. Its inhibition was on par with DNase I treatment at specific doses. Thus, ALP may play an important role in the biofilm formation. Likewise, ALP inhibition may be a novel target for anti-biofilm therapeutics.

References

Jul 1, 1979·The Biochemical Journal·L E Seargeant, R A Stinson
Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·J E Coleman
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P HenthornT Kadesch
Jul 1, 1974·Japanese Journal of Microbiology·K OkabayashiD Mizuno
Mar 19, 1999·Emerging Infectious Diseases·R J RubinA Moiduddin
Apr 7, 2006·Applied and Environmental Microbiology·Cordula LembkeBernd Kreikemeyer
Dec 19, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Garth A JamesPhilip S Stewart
Apr 21, 2009·Applied and Environmental Microbiology·Akinobu ItoSatoshi Okabe
Feb 13, 2010·International Journal of Antimicrobial Agents·Niels HøibyOana Ciofu
Aug 24, 2011·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Afreenish HassanMuhammad Iqbal
Aug 8, 2013·PloS One·Eric J KalivodaRobert M Q Shanks
Oct 15, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Khang T NguyenRobert D Galiano
May 20, 2014·International Journal of Medical Microbiology : IJMM·Yibo YouBaolin Sun
Mar 7, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·U Venkateswara PrasadP V G K Sarma
Apr 17, 2016·The Journal of Surgical Research·Jason Fawley, David M Gourlay
Apr 20, 2016·Journal of Environmental Sciences (China)·Shuangshuang LiDunhai Li

❮ Previous
Next ❯

Citations

Jun 12, 2021·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Beatriz BögerKlaus Fischer
Jan 12, 2021·Journal of the American Chemical Society·Adam C SedgwickJonathan L Sessler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

The Journal of Surgical Research
Donavon J HessCarol L Wells
Nihon rinsho. Japanese journal of clinical medicine
Shiro Iino
Nihon rinsho. Japanese journal of clinical medicine
S Iino
© 2022 Meta ULC. All rights reserved