Jan 1, 1962

Alkaline phosphatase content and the effects of prednisolone on mammalian cells in culture

The Journal of General Physiology
R P Cox, C M MACLEOD

Abstract

The alkaline phosphatase content of different tissue culture cell lines has been shown to vary from no detectable activity to high enzyme concentration. Within the epithelial lines studied alkaline phosphatase is either constitutive or inducible. Two epithelial cell strains in which alkaline phosphatase was "absent" could be induced to develop significant amounts of the enzyme when grown in the presence of Delta(1)-hydrocortisone. Phosphate did not repress enzyme induction by prednisolone. Under conditions of deadaptation the induced enzyme was diluted by cell multiplication. The mouse fibroblastic L line and several human fibroblastic lines did not contain alkaline phosphatase when grown under the conditions described nor could they be induced to produce the enzyme when cultivated in medium with prednisolone. Delta(1)-Hydrocortisone has other characteristic effects on established mammalian cell cultures which vary among cell lines. Human epithelial lines show reduction in cell multiplication with increase in mitotic index. The cytoplasm is increased and cell volume is nearly doubled. Mouse fibroblasts show a similar reduction in cell multiplication with a decrease in mitotic index. There is no increase in cell cytoplasm. Human...Continue Reading

  • References26
  • Citations55

References

Mentioned in this Paper

Specimen Type - Fibroblasts
Phosphoric Monoester Hydrolases
Predonine
Immune Sera
Alkaline Phosphatase Measurement
Prednisolone
Phosphate Measurement
Alkaline Phosphatase
Cell Proliferation
Alkaline Phosphatase Activity

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