Jul 1, 1976

Alkaline phosphatase. I. Kinetics and inhibition by levamisole of purified isoenzymes from humans

Clinical Chemistry
H Van Belle

Abstract

I studied the kinetics and sensitivity toward inhibition by levamisole and R 8231 of the most important human alkaline phosphatase isoenzymes. N-Ethylaminoethanol proved superior to the now widely used diethanolamine buffer, especially for the enzymes from the intestine and placenta, behaving as an uncompetitive activator. The optimum pH largely depends on the substrate concentration. The addition of Mg2+ has no effect on the activities. The meaning of Km-values for alkaline phosphatases is questioned. Isoenzymes from human liver, bone, kidney, and spleen are strongly inhibited by levamisole or R 8231 at concentrations that barely affect the enzymes from intestine or placenta. The inhibition is stereospecific, uncompetitive, and not changed by Mg2+. Inhibition is counteracted by increasing concentrations of N-ethylaminoethanol. The mechanism of inhibition is suggested to be formation of a complex with the phosphoenzyme.

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Mentioned in this Paper

Enzymes, antithrombotic
Placenta Specimen
Spleen
Tissue Specificity
Diethanolamine
Alkaline Phosphatase Measurement
Alkaline phosphatase isoenzyme
Placenta
R 8231
Alkaline Phosphatase

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