PMID: 821842Mar 1, 1976

Alkaline phosphatase of human and calf small intestine. Purification and immunochemical characterization

Hoppe-Seyler's Zeitschrift für physiologische Chemie
M Khattab, G Pfleiderer

Abstract

Alkaline phosphatase from human and calf small intestines has been prepared and purified until homogeneous, as judged by polyacrylamide gel electrophoresis, by means of the following techniques: n-butanol extraction, ammonium sulfate precipitation, acetone fractionation, ion exchange chromatography and isoelectric focusing in a sucrose density gradient. Three and two alkaline phosphatase froms from human and calf small intestines, respectively could be isolated by preparative isolectric focusing. The relative amounts of these components are not constant, but they have the same catalytic properties, suggesting that they may embody a common protein core with an identical active centre(s). Precipitating antisera for alkaline phosphatase from human and calf intestine have been prepard in rabbits by intramuscular, dermal, subcutaneous and intravenous administration of the pure major component of each enzyme species. Both antisera precipitate completely their homologous as well as their heterologous antigens (intestinal enzyme) and showed partial identity with placental alkaline phosphatase. There was no reaction with alkaline phosphatase from bone, liver heart, spleen, lung, stomach, pancreas, brain, bile-gall bladder and erythrocyt...Continue Reading

References

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Citations

Jan 1, 1987·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·K W Gasser, L B Kirschner
Jan 15, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Harris
Jan 1, 1984·The International Journal of Biochemistry·G G ChangF Pan
Aug 1, 1985·Clinical Biochemistry·W C GriffithsG Horvath
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R A MulivorH Harris
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F J Benham, H Harris
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D J GoldsteinH Harris
Sep 15, 1980·Klinische Wochenschrift·F G Lehmann
Mar 1, 1982·Critical Reviews in Clinical Laboratory Sciences·P M Crofton
Jun 1, 1981·Clinical Biochemistry·K CavanaghH F Fackrell
May 7, 2014·Journal of Pharmaceutical Sciences·Koreyoshi ImamuraKazuhiro Nakanishi
Jan 1, 1982·Preparative Biochemistry·J Kirchberger, G Kopperschläger

Related Concepts

Alkaline Phosphatase
Bos indicus
Immunodiffusion Measurement
Immunoelectrophoresis
Intestines, Small
Alloenzymes
Neuraminidase
Tissue Specificity
Placenta

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