Alkaline unfolding and salt-induced folding of arginine kinase from shrimp Feneropenaeus chinensis under high pH conditions

International Journal of Biological Macromolecules
Wei-Qun LiuZhen-Hang Yu

Abstract

The structural and functional properties of arginine kinase (AK) in alkaline conditions in the absence or presence of salt have been investigated. The conformational changes of AK during alkaline unfolding and salt-induced folding at alkaline pH were monitored using intrinsic fluorescence emission, binding of the fluorescence probe 1-anilino-8-naphthalenesulfonate and circular dichroism. The results for the alkaline unfolded enzyme showed that much lower pH (11.0) was required to cause the complete loss of AK activity than was required to cause an obvious conformational change of the enzyme. Compared with the completely unfolded state in 5M urea, the high pH denatured enzyme had some residual secondary and tertiary structure even at pH 13.0. Increasing the ionic strength by adding salt at pH 12.75 resulted in the formation of a relatively compact tertiary structure and a little new secondary structure with hydrophobic surface enhancement. These results indicate that the partially folded state formed under alkaline conditions may have similarities to the molten globule state which is compact, but it has a poorly defined tertiary structure and a native-like secondary structure.

References

Aug 1, 1975·The Biochemical Journal·E O Anosike, D C Watts
Jul 22, 1991·FEBS Letters·O B Ptitsyn
Nov 2, 1987·FEBS Letters·R I Gilmanshin, O B Ptitsyn
Jun 18, 1974·Biochemistry·K P Wong, L M Hamlin
Feb 11, 1969·Biochimica Et Biophysica Acta·Y RobinN V Thoai
Jan 2, 1984·FEBS Letters·D A DolgikhO B Ptitsyn
Feb 1, 1996·Archives of Biochemistry and Biophysics·R M France, S H Grossman
Jan 1, 1994·Enzyme & Protein·M Q Huang, H M Zhou
Jun 1, 1996·International Journal of Peptide and Protein Research·W P LeH M Zhou
Sep 1, 1997·Archives of Biochemistry and Biophysics·R M FranceS H Grossman
Aug 6, 1998·European Journal of Biochemistry·S PrajapatiS K Jain
Sep 8, 1998·International Journal of Biological Macromolecules·J H BaiH M Zhou
Oct 1, 1958·Physiological Reviews·A H ENNOR, J F MORRISON

❮ Previous
Next ❯

Citations

Feb 26, 2009·Fish & Shellfish Immunology·Cui-Luan YaoJian-Hai Xiang
Aug 4, 2007·Chembiochem : a European Journal of Chemical Biology·Yousef AhmadibeniKeykavous Parang
Nov 22, 2011·Molecular Biology Reports·Chengwen SongShuangyan Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.