Alkaloid cluster gene ccsA of the ergot fungus Claviceps purpurea encodes chanoclavine I synthase, a flavin adenine dinucleotide-containing oxidoreductase mediating the transformation of N-methyl-dimethylallyltryptophan to chanoclavine I.

Applied and Environmental Microbiology
Nicole LorenzP Tudzynski

Abstract

Ergot alkaloids are indole-derived secondary metabolites synthesized by the phytopathogenic ascomycete Claviceps purpurea. In wild-type strains, they are exclusively produced in the sclerotium, a hibernation structure; for biotechnological applications, submerse production strains have been generated by mutagenesis. It was shown previously that the enzymes specific for alkaloid biosynthesis are encoded by a gene cluster of 68.5 kb. This ergot alkaloid cluster consists of 14 genes coregulated and expressed under alkaloid-producing conditions. Although the role of some of the cluster genes in alkaloid biosynthesis could be confirmed by a targeted knockout approach, further functional analyses are needed, especially concerning the early pathway-specific steps up to the production of clavine alkaloids. Therefore, the gene ccsA, originally named easE and preliminarily annotated as coding for a flavin adenine dinucleotide-containing oxidoreductase, was deleted in the C. purpurea strain P1, which is able to synthesize ergot alkaloids in axenic culture. Five independent knockout mutants were analyzed with regard to alkaloid-producing capability. Thin-layer chromatography (TLC), ultrapressure liquid chromatography (UPLC), and mass spect...Continue Reading

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Jan 23, 2015·Toxins·Sarah L Robinson, Daniel G Panaccione
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Apr 3, 2012·Current Opinion in Plant Biology·Aiko TanakaBarry Scott
Jan 21, 2012·Biotechnology Advances·Helena HulvováIvo Frébort
May 25, 2016·Organic & Biomolecular Chemistry·Stephanie R McCabe, Peter Wipf
Jan 13, 2015·Angewandte Chemie·Hsiao-Ching LinYi Tang
Aug 29, 2014·Natural Product Reports·Dorota JakubczykSarah E O'Connor
Aug 5, 2018·Applied and Environmental Microbiology·Samantha J FabianDaniel G Panaccione
Jun 21, 2020·World Journal of Microbiology & Biotechnology·R BharadwajS Ramalingam
Feb 9, 2017·Phytopathology·Simona FloreaChristopher L Schardl
Apr 29, 2021·Chemical Society Reviews·Cooper S JamiesonJohn M Billingsley
Apr 4, 2020·Organic Letters·Kuan-Lin ChenHsiao-Ching Lin
Oct 18, 2019·Journal of the American Chemical Society·Yongpeng YaoShu-Shan Gao

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