PMID: 11909625Mar 23, 2002Paper

Alkaloid production in Duboisia hybrid hairy root cultures overexpressing the pmt gene

Phytochemistry
Elisabeth MoyanoM T Piñol

Abstract

Putrescine:SAM N-methyltransferase (PMT) catalyses the N-methylation of the diamine putrescine to form N-methylputrescine, the first specific precursor of both tropane and pyridine-type alkaloids, which are present together in the roots of Duboisia plants. The pmt gene of Nicotiana tabacum was placed under the regulation of the CaMV 35S promoter and introduced into the genome of a scopolamine-rich Duboisia hybrid by a binary vector system using the disarmed Agrobacterium tumefaciens strain C58C1 carrying the rooting plasmid pRiA4. The presence of the foreign gene in kanamycin-resistant hairy roots and its overexpression were confirmed by polymerase chain reaction and Northern blot analysis respectively. The N-methylputrescine levels of the resulting engineered hairy roots increased (2-4-fold) compared to wild type roots, but there was no significant increase in either tropane or pyridine-type alkaloids.

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Citations

Jul 5, 2007·Plant Cell Reports·Sonia Gómez-GaleraPaul Christou
Mar 11, 2010·Plant Cell Reports·Nita SachanDeane L Falcone
Apr 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lei ZhangKexuan Tang
Jun 23, 2012·Phytochemistry·Geoffrey A Cordell
Aug 5, 2009·Phytochemistry·Stefan BiastoffBirgit Dräger
Aug 25, 2010·Biotechnology and Applied Biochemistry·Shakti MehrotraArun Kumar Kukreja
Aug 15, 2006·Journal of Plant Physiology·Elisabeth MoyanoM Teresa Piñol
Jul 30, 2003·Journal of Experimental Botany·Grit RotheBirgit Dräger
Sep 17, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Javier PalazónMohammad Hossein Mirjalili
Mar 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kathrin Laura Kohnen-Johannsen, Oliver Kayser

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