PMID: 9546151Apr 18, 1998Paper

Alkane hydroxylase from Acinetobacter sp. strain ADP1 is encoded by alkM and belongs to a new family of bacterial integral-membrane hydrocarbon hydroxylases

Applied and Environmental Microbiology
A RatajczakW Hillen

Abstract

Degradation of long-chain alkanes by Acinetobacter sp. strain ADP1 involves rubredoxin and rubredoxin reductase. We complemented a mutant deficient in alkane utilization and sequenced four open reading frames (ORFs) on the complementing DNA. Each of these ORFs was disrupted by insertional mutagenesis on the chromosome. As determined from sequence comparisons, ORF1 and ORF4 seem to encode a rotamase of the PpiC type and an acyl coenzyme A dehydrogenase, respectively. Disruption of these ORFs does not affect alkane utilization. In contrast, the two other ORFs, alkR and alkM, are essential for growth on alkanes as sole carbon sources. alkR encodes a polypeptide with extensive homology to AraC-XyIS-like transcriptional regulators. It is located next to alkM, which encodes the terminal alkane hydroxylase, but is in the opposite orientation. Sequence homologies with other bacterial integral-membrane hydrocarbon hydroxylases suggest that AlkM may be the first member of a new protein family. The genes identified here are not linked to the rubredoxin- and rubredoxin reductase-encoding genes on the Acinetobacter sp. strain ADP1 chromosome.

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Citations

Feb 24, 2001·Environmental Microbiology·T H SmitsJ B van Beilen
Oct 27, 2001·Applied and Environmental Microbiology·N HamamuraD J Arp
Aug 2, 2005·FEMS Microbiology Letters·Luis A Sayavedra-SotoDaniel J Arp
Mar 18, 2006·FEMS Microbiology Ecology·Roger MarchantAmedea Perfumo
Sep 6, 2000·Journal of Applied Microbiology·A Vomberg, U Klinner
Nov 29, 2005·Environmental Microbiology·Dick B JanssenPeter Terpstra
Dec 4, 2002·Environmental Microbiology·Jan B van BeilenBernard Witholt
Feb 20, 2015·Applied Microbiology and Biotechnology·Jaejoon Jung, Woojun Park
Dec 10, 2013·Antonie van Leeuwenhoek·Ji-Quan SunXiao-Lei Wu
May 19, 2006·Journal of Bacteriology·Julia S SabirovaPeter N Golyshin
Aug 29, 2018·Biotechnology for Biofuels·Tapio LehtinenVille Santala
Mar 24, 2017·FEMS Microbiology Letters·Tapio LehtinenSuvi Santala
Mar 27, 2021·Essays in Biochemistry·Suvi Santala, Ville Santala
Dec 19, 2020·Biotechnology Advances·Laura K MartinIan P Thompson
Aug 17, 1999·Current Opinion in Biotechnology·S W May
Jun 6, 2003·Applied and Environmental Microbiology·R MargesinL G Whyte
May 6, 2003·Journal of Bacteriology·Mercedes M MarínFernando Rojo
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jonathan D Van HammeOwen P Ward
Mar 2, 2002·Journal of Bacteriology·Theo H M SmitsJan B van Beilen
Mar 13, 2002·Journal of Bacteriology·Alisa S VangnaiLuis A Sayavedra-Soto
Feb 17, 2012·ACS Synthetic Biology·Suvi SantalaVille Santala

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