All-Optical Assay to Study Biological Neural Networks

Frontiers in Neuroscience
Wardiya Afshar SaberMaciej Antkowiak

Abstract

We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetic construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (GECI) (jRCaMP1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. Additionally, we have developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity.

References

Jan 1, 1995·Biosensors & Bioelectronics·G W GrossM C Wu
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
Aug 24, 2005·Nature Neuroscience·Edward S BoydenKarl Deisseroth
Mar 24, 2006·Journal of Neuroscience Methods·Katherine L Perkins
Oct 14, 2008·Methods : a Companion to Methods in Enzymology·Janet E McCombs, Amy E Palmer
Dec 17, 2008·Nature Neuroscience·André BerndtKarl Deisseroth
May 6, 2009·Biophysical Journal·Stephan JunekDetlev Schild
Oct 16, 2009·Nature·Massimo Scanziani, Michael Häusser
Jun 22, 2010·Experimental Physiology·Philipp SchoenenbergerThomas G Oertner
Apr 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·André BerndtThomas G Oertner
Mar 13, 2012·Neuron·Christine Grienberger, Arthur Konnerth
Mar 21, 2012·Nature Reviews. Neuroscience·Kay M Tye, Karl Deisseroth
Jun 26, 2012·Neuron·A Paul AlivisatosRafael Yuste
Oct 5, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jasper AkerboomLoren L Looger
Feb 5, 2013·Cold Spring Harbor Protocols·Martin D BootmanMichael Sanderson
May 18, 2013·Methods in Molecular Biology·Baojin Ding, Daniel L Kilpatrick
Jun 27, 2013·Nature Neuroscience·Adam M PackerMichael Häusser
Oct 11, 2013·Cerebral Cortex·Karlijn I van AerdeDirk Feldmeyer
Feb 11, 2014·Nature Methods·Nathan C KlapoetkeEdward S Boyden
Jun 24, 2014·Nature Methods·Daniel R HochbaumAdam E Cohen
Jan 30, 2015·Journal of Neuroscience Methods·Tapan P PatelDavid F Meaney
Apr 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Cedric BardyFred H Gage
Aug 30, 2016·Nature Neuroscience·Michael Z Lin, Mark J Schnitzer
Feb 15, 2017·ACS Chemical Biology·Jung-Hwa ChoRobert H Chow
Mar 9, 2017·Current Opinion in Biotechnology·Viviana Agus, Harald Janovjak
Mar 18, 2017·Nature Reviews. Neuroscience·Christina K KimKarl Deisseroth
Sep 22, 2017·ENeuro·Nicholas A SteinmetzKenneth D Harris
Nov 23, 2017·Frontiers in Physiology·Susann BjörkEero Mervaala
Jan 23, 2018·Frontiers in Pharmacology·Amber KerkhofsHuibert D Mansvelder

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Citations

Sep 21, 2018·Frontiers in Neuroscience·Alastair I GraingerEric J Hill
Feb 23, 2020·Molecular Autism·Wardiya Afshar Saber, Mustafa Sahin

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Methods Mentioned

BETA
fluorescence microscopy
PCR
Transfection
transgenic

Software Mentioned

LabVIEW
NeuroCa
Andor Solis
mRuby
BrainPhys
Matlab
FluoroSNNAP
OptoCaMP

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