All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors.

Frontiers in Cellular Neuroscience
F. PuppoAnna Devor

Abstract

Voltage imaging and "all-optical electrophysiology" in human induced pluripotent stem cell (hiPSC)-derived neurons have opened unprecedented opportunities for high-throughput phenotyping of activity in neurons possessing unique genetic backgrounds of individual patients. While prior all-optical electrophysiology studies relied on genetically encoded voltage indicators, here, we demonstrate an alternative protocol using a synthetic voltage sensor and genetically encoded optogenetic actuator that generate robust and reproducible results. We demonstrate the functionality of this method by measuring spontaneous and evoked activity in three independent hiPSC-derived neuronal cell lines with distinct genetic backgrounds.

References

Feb 25, 1998·IEEE Transactions on Bio-medical Engineering·S Mukhopadhyay, G C Ray
Jul 20, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·R SegevE Ben-Jacob
Feb 9, 2006·BMC Neuroscience·Daniel A WagenaarSteve M Potter
May 22, 2007·Nature Reviews. Neuroscience·Bruce P Bean
Nov 22, 2007·Science·Junying YuJames A Thomson
Sep 26, 2009·Neuron·Eran A MukamelMark J Schnitzer
Oct 22, 2010·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Hugo H HansonGreg R Phillips
Jan 12, 2011·Neuron·Darcy S PeterkaRafael Yuste
Apr 15, 2011·Nature·Kristen J BrennandFred H Gage
Jun 22, 2012·Current Opinion in Neurobiology·Thanathom ChailangkarnAlysson Renato Muotri
Jun 24, 2014·Nature Methods·Daniel R HochbaumAdam E Cohen
Nov 12, 2014·Molecular Psychiatry·K Griesi-OliveiraA R Muotri
Aug 4, 2015·Journal of the American Chemical Society·Yi-Lin HuangEvan W Miller
Aug 27, 2015·Nature Neuroscience·Karl Deisseroth
Oct 16, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Valentina EmilianiMichael Häusser
Mar 6, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zhen-Ning ZhangAdah Almutairi
Mar 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yaron PennElisha Moses
Jun 20, 2016·Current Opinion in Chemical Biology·Evan W Miller
Jul 6, 2016·Molecular Psychiatry·Maria C MarchettoAlysson R Muotri
Sep 30, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Helen H Yang, François St-Pierre
Jan 7, 2017·Optics Express·Reto Fiolka
Apr 25, 2017·Nature Communications·James J FinkEric S Levine
Jul 27, 2017·Biochemistry·Rishikesh U Kulkarni, Evan W Miller
Sep 12, 2017·Current Protocols in Pharmacology·Christopher A WerleyGraham T Dempsey
Sep 7, 2019·Cell·Lilia M IakouchevaJonathan Sebat
Nov 7, 2019·Nature·Alexander D Reyes

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