PMID: 8592069Jan 1, 1996Paper

all-trans-retinoic acid regulates retinol and 3,4-didehydroretinol metabolism in cultured human epidermal keratinocytes

The Journal of Investigative Dermatology
R K Randolph, M Simon

Abstract

The potential for all-trans-retinoic acid to regulate the metabolism of 3H-retinol and 3H-3,4-didehydroretinol was examined in cultured human epidermal keratinocytes. Confluent cultures were treated daily with medium containing 5% fetal bovine serum or the same medium supplemented with nanomolar concentrations of all-trans-retinoic acid for up to 3 d. During the last 24 of treatment, cells were incubated with 3H-retinol or 3H-3,4-didehydroretinol for 24 h (isotopic steady state) to label the endogenous retinoids. After the labeling period, one group of cells was harvested and another group was allowed to incubate for an additional 24 h in the absence of medium retinol for the determination of endogenous 3H-retinoid utilization. The 3H-retinoids present in cells were extracted and quantitated by reverse-phased high-pressure liquid chromatography. Keratinocytes treated with retinoic acid and labeled with 3H-retinol exhibited time- and concentration-dependent (i) increases in retinyl ester mass, (ii) increases in the rate of retinyl ester synthesis, (iii) decreases in retinyl ester utilization, and (iv) decreases in the cellular concentrations of retinoic and 3,4-didehydroretinoic acids. There was no effect of exogenous retinoic a...Continue Reading

References

Jan 1, 1991·Skin Pharmacology : the Official Journal of the Skin Pharmacology Society·H TörmäA Vahlquist
Dec 1, 1990·The Journal of Cell Biology·E Fuchs
Jan 1, 1990·Methods in Enzymology·A B Barua
Mar 1, 1988·International Journal of Dermatology·A Vahlquist, H Törmä
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Mar 1, 1993·Archives of Biochemistry and Biophysics·T Matsuura, A C Ross

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Citations

Jan 21, 2021·Nutrients·Helen B Everts, Eleonore-Nausica Akuailou
Oct 16, 1999·Biochimica Et Biophysica Acta·J L Napoli
Sep 18, 1998·The Journal of Investigative Dermatology·R K Randolph, M Simon
Mar 6, 2003·Molecular Genetics and Metabolism·Nedialka G MarkovaM Simon

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