PMID: 7540854Jun 1, 1995Paper

All-trans retinoic acid shows multiple effects on the survival, proliferation and differentiation of human fetal CD34+ haemopoietic progenitor cells

British Journal of Haematology
G ZauliS Capitani

Abstract

To evaluate the effect of all-trans retinoic acid (RA) on fetal haemopoiesis, we performed serum-free liquid and semisolid cultures using CD34+ cells purified from midtrimester human fetal blood samples. RA, at both physiological (10(-11) and 10(-12)M) and pharmacological (10(-6) and 10(-7)M) concentrations, significantly (P < 0.01) promoted the survival of fetal CD34+ cells in liquid cultures from day 3 onwards, by suppressing apoptosis induced by serum and growth factor deprivation. On the other hand, RA alone had no significant effect on the proliferation and differentiation of fetal haemopoietic progenitors. In the presence of optimal concentrations of recombinant interleukin-3 (IL-3), stem cell factor (SCF), granulocyte/macrophage-colony stimulating factor (GM-CSF), and erythropoietin (Epo), low and high doses of RA induced striking differential effects on CD34+ cell proliferation in liquid cultures and colony formation in semisolid assays. In fact, 10(-11)M and 10(-12)M RA were able to: (i) significantly (P < 0.05) increase 3H-thymidine uptake by fetal CD34+ cells in liquid cultures, and (ii) variably promote the growth of pluripotent (CFU-GEMM, P < 0.05), early (BFU-meg) and late (CFU-meg, P < 0.01) megakaryocyte, granul...Continue Reading

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Citations

Jul 9, 2002·British Journal of Haematology·Olivier HeraultChristian Binet
Jan 24, 2004·Leukemia & Lymphoma·Tal OrenTodd Evans
Jun 19, 2003·British Journal of Pharmacology·V Moreno-ManzanoF J Lucio Cazana
May 15, 2012·British Journal of Pharmacology·M R CarratùA Vacca
Dec 30, 2014·Molecular Aspects of Medicine·Alessandra di MasiClara Nervi
Jul 28, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jin Seuk KimDuk-Hwan Kim
Aug 9, 2005·Biochemical and Biophysical Research Communications·Joong Ho AhnJong Woo Chung
Jan 6, 2015·International Journal of Cancer. Journal International Du Cancer·Rihab R NasrNadine D Darwiche
Apr 20, 2002·European Journal of Clinical Nutrition·R D Semba, M W Bloem
Jul 10, 1999·The Journal of Biological Chemistry·V Moreno-ManzanoM Kitamura
Nov 13, 2001·Oncogene·P Kastner, S Chan
Jun 19, 2004·Cancer Research·Zhongxing LiangHyunsuk Shim

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