PMID: 7536299Jan 1, 1994Paper

All you wanted to know about SELEX

Molecular Biology Reports
S J Klug, M Famulok

Abstract

In vitro selection, or SELEX, is a technique that allows the simultaneous screening of highly diverse pools of different RNA or DNA (dsDNA or ssDNA) molecules for a particular feature. Different examples from a great variety of applications of in vitro selection experiments are described and a detailed overview of the method and its variations will be given. Some especially conclusive in vitro selection experiments are discussed in detail to illustrate the potential power and diversity of this method. Potential restrictions of the methods and possible ways to overcome them are pointed out.

References

Apr 28, 1992·Biochemistry·T Pan, O C Uhlenbeck
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C TuerkL Gold
Mar 1, 1992·Trends in Biochemical Sciences·J W Szostak
Dec 5, 1992·Journal of Molecular Biology·D SchneiderL Gold
Aug 1, 1992·PCR Methods and Applications·R C Cadwell, G F Joyce
Jul 31, 1992·Science·A A Beaudry, G F Joyce
Aug 30, 1990·Nature·A D Ellington, J W Szostak
Jan 1, 1990·Journal of Immunoassay·S M PonceletD Collet-Cassart
Dec 7, 1989·Nature·M L Zapp, M R Green
Jan 1, 1983·Pharmacotherapy·L Hendeles, M Weinberger
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D JellinekN Janjić
Feb 1, 1994·Biochemistry·J R Lorsch, J W Szostak
Mar 11, 1994·Science·R D JenisonB Polisky
Jul 11, 1994·Nucleic Acids Research·M F KubikD Tasset
May 1, 1994·Current Biology : CB·A D Ellington
Jan 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D SchneiderT Platt
Aug 5, 1993·Nature·M Sassanfar, J W Szostak
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E Szathmáry
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R F MacayaJ Feigon
Jun 1, 1993·Biochemistry·G J ConnellM Yarus
May 25, 1993·Nucleic Acids Research·K Harada, L E Orgel
Aug 20, 2010·Cold Spring Harbor Perspectives in Biology·Michael Yarus

❮ Previous
Next ❯

Citations

Jun 6, 2000·Advanced Drug Delivery Reviews·E MastrobattistaG Storm
Feb 24, 2001·Biosensors & Bioelectronics·S S IqbalJ P Chambers
Aug 19, 1999·Biosensors & Bioelectronics·J G Bruno, J L Kiel
Jun 1, 1996·Current Opinion in Structural Biology·K W UphoffA D Ellington
Aug 1, 1996·Chemistry & Biology·J FeigonF W Smith
Jun 28, 2011·Nature Biotechnology·Razvan NutiuChristopher B Burge
Mar 13, 2013·Nature Reviews. Genetics·Michaela Müller-McNicoll, Karla M Neugebauer
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Seunghee LeeSoo-Kyung Lee
Dec 17, 2010·Bioinformatics·Melissa S Cline, Rachel Karchin
Nov 1, 2000·Nucleic Acids Research·S Nole-Wilson, B A Krizek
Dec 20, 2002·Nucleic Acids Research·Michiyasu ItohJun-ichi Fujisawa
Apr 25, 2003·Nucleic Acids Research·Irina Calin-Jageman, Allen W Nicholson
Dec 19, 2003·Nucleic Acids Research·Jennifer F LeeAndrew D Ellington
Feb 1, 2005·Nucleic Acids Research·Maris ZivartsRonald R Breaker
May 10, 2007·Nucleic Acids Research·Hugh P MorganMalcolm D Walkinshaw
Jan 25, 2006·Journal of Nanoscience and Nanotechnology·Peixuan Guo
Nov 23, 2006·BMC Bioinformatics·Venkata ThodimaYouping Deng
Sep 27, 2006·RNA·Martina HalleggerMichael F Jantsch
Jun 12, 2013·PloS One·Wataru YoshidaKazunori Ikebukuro
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·S J KlugM Famulok
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·S M RinkL A Loeb
Aug 9, 2005·FEBS Letters·Rasa Beinoraviciūte-KellnerGerhard Krauss
Jan 24, 2012·FEBS Letters·Cristiano ChiarabelliPier Luigi Luisi
May 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Mingxu YouSamie R Jaffrey
Aug 31, 2016·Topics in Current Chemistry·Stephanie Kath-Schorr
Jun 9, 2009·Protein Expression and Purification·Pinar CalikTunçer H Ozdamar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.