Allele- and tir-independent functions of intimin in diverse animal infection models.

Frontiers in Microbiology
Emily M MallickDavid B Schauer

Abstract

Upon binding to intestinal epithelial cells, enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. coli (EPEC), and Citrobacter rodentium trigger formation of actin pedestals beneath bound bacteria. Pedestal formation has been associated with enhanced colonization, and requires intimin, an adhesin that binds to the bacterial effector translocated intimin receptor (Tir), which is translocated to the host cell membrane and promotes bacterial adherence and pedestal formation. Intimin has been suggested to also promote cell adhesion by binding one or more host receptors, and allelic differences in intimin have been associated with differences in tissue and host specificity. We assessed the function of EHEC, EPEC, or C. rodentium intimin, or a set of intimin derivatives with varying Tir-binding abilities in animal models of infection. We found that EPEC and EHEC intimin were functionally indistinguishable during infection of gnotobiotic piglets by EHEC, and that EPEC, EHEC, and C. rodentium intimin were functionally indistinguishable during infection of C57BL/6 mice by C. rodentium. A derivative of EHEC intimin that bound Tir but did not promote robust pedestal formation on cultured cells was unable to promote C. rodentium ...Continue Reading

Citations

Oct 30, 2014·Molecular Microbiology·Jack C LeoDirk Linke
Jan 19, 2015·International Journal of Medical Microbiology : IJMM·Jack C LeoDirk Linke
Oct 9, 2012·The Journal of Clinical Investigation·Emily M MallickDavid B Schauer

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BETA
PCR
Protein Assay

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