Allele-specific methylation of imprinted genes in fetal cord blood is influenced by cis-acting genetic variants and parental factors

Epigenomics
Ramya PotabattulaThomas Haaf

Abstract

To examine the effects of genetic variation, parental age and BMI on parental allele-specific methylation of imprinted genes in fetal cord blood samples. We have developed SNP genotyping and deep bisulphite sequencing assays for six imprinted genes to determine parental allele-specific methylation patterns in diploid somatic tissues. Multivariate linear regression analyses revealed a negative correlation of paternal age with paternal MEG3 allele methylation in fetal cord blood. Methylation of the maternal PEG3 allele showed a positive correlation with maternal age. Paternal BMI was positively correlated with paternal MEST allele methylation. In addition to parental origin, allele-specific methylation of most imprinted genes was largely dependent on the underlying SNP haplotype. Our study supports the idea that parental factors can have an impact, although of small effect size, on the epigenome of the next generation, providing an additional layer of complexity to phenotypic diversity.

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Citations

Mar 27, 2020·Frontiers in Genetics·Yasmeen SalamehNady El Hajj
Feb 1, 2021·International Journal of Epidemiology·Gemma C SharpUNKNOWN 36 other members of the Pregnancy and Childhood Epigenetics (PACE) consortium

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Methods Mentioned

BETA
genotyping
Assay
PCRs
PCR

Software Mentioned

Amplikyzer2
PyroMark Assay Design
Amplikyzer
Pyro Q - CpG
Illumina Genome Analyzer
R
R scripts
house

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