PMID: 9431955Feb 12, 1998Paper

Allele-specific PCR method based on pncA and oxyR sequences for distinguishing Mycobacterium bovis from Mycobacterium tuberculosis: intraspecific M. bovis pncA sequence polymorphism

Journal of Clinical Microbiology
L E Espinosa de los MonterosJ Blázquez

Abstract

An allele-specific amplification method based on two genetic polymorphisms to differentiate Mycobacterium tuberculosis from Mycobacterium bovis was tested. Based on the differences found at position 169 in the pncA genes from M. tuberculosis and M. bovis, a PCR system which was able to differentiate most of the 237 M. tuberculosis complex isolates tested in one of the two species was developed. All 121 M. tuberculosis strains showed the expected base (cytosine) at position 169. Most of the M. bovis isolates had a guanine at the cited position. Nevertheless, 18 of the 116 M. bovis isolates, all of them goat isolates, showed the pncA polymorphism specific to M. tuberculosis. These results suggest that goat M. bovis may be the nicotinamidase-missing link at the origin of the M. tuberculosis species. Based on the polymorphism found at position 285 in the oxyR gene, the same system was used to differentiate M. tuberculosis from M. bovis. In this case, DNAs from all 121 M. tuberculosis isolates had the expected base (guanine) at this position. In addition, all 116 M. bovis isolates, including those from goats, showed the identical polymorphism (adenine). The oxyR allele-specific amplification method can differentiate M. bovis from M....Continue Reading

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Citations

Sep 18, 2003·International Journal of Systematic and Evolutionary Microbiology·Debby V CousinsAmelia Bernardelli
Nov 24, 2011·Case Reports in Medicine·Sílvia BacalhauJoão Farela Neves
Apr 13, 2012·Human Vaccines & Immunotherapeutics·Nadya MarkovaLilia Michailova
Aug 22, 2007·Memórias do Instituto Oswaldo Cruz·A F C NassarR A Ogata
Jan 26, 2021·PLoS Neglected Tropical Diseases·Thoko Flav KapalamulaChie Nakajima
May 17, 2014·Research in Veterinary Science·E GormleyS Rodriguez-Campos
Mar 29, 2001·American Journal of Physical Anthropology·S MaysG Turner-Walker
Oct 7, 2005·Journal of Clinical Microbiology·Wolfgang M ProdingerIrmgard Moser
Nov 1, 2002·Journal of Clinical Microbiology·Dawit KidaneMorten Harboe
Jan 9, 2003·Journal of Clinical Microbiology·Albert R ZinkAndreas G Nerlich
Jun 7, 2003·Journal of Clinical Microbiology·Sara Ngo Niobe-EyangohM Cristina Gutierrez
Sep 17, 2010·Journal of Clinical Microbiology·Suheir EreqatZiad Abdeen
Feb 7, 2001·Journal of Clinical Microbiology·M M HannanJ T Crawford
May 15, 1999·Journal of Clinical Microbiology·R FrothinghamD L Williams
Jun 12, 1999·Journal of Clinical Microbiology·J G RodríguezA Cataldi
May 31, 2002·Journal of Clinical Microbiology·Wolfgang M ProdingerWalter Glawischnig
Aug 31, 2002·Journal of Clinical Microbiology·Johanna MäkinenHanna Soini
Mar 18, 2004·Microbes and Infection·Tuija KoivulaGunilla Källenius
Nov 2, 2021·Transboundary and Emerging Diseases·Tai-Hua ChanRuwen Jou

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