Alleles of the bovine DGAT1 variable number of tandem repeat associated with a milk fat QTL at chromosome 14 can stimulate gene expression

Physiological Genomics
Rainer FürbassC Kühn

Abstract

A quantitative trait locus (QTL) affecting milk fat percentage has been mapped to the centromeric end of the bovine chromosome 14 (BTA14). This genomic area includes the DGAT1 gene, which encodes acyl-CoA:diacylglycerol acyltransferase 1, the key enzyme of triglyceride biosynthesis. Genetic and biochemical studies led to the identification of the nonconservative DGAT1-K232A polymorphism as a causal mutation for the QTL. In addition to this, another polymorphism in the 5'-regulatory region of this gene, the DGAT1 variable number of tandem repeat (VNTR), also showed a strong association with milk fat percentage. This promoter VNTR polymorphism affects the number of potential Sp1 binding sites and therefore might have an impact on DGAT1 expression and also milk fat content. Hence, the DGAT1 VNTR polymorphism might be another causal mutation for the BTA14 QTL. However, evidence for Sp1 binding to this polymorphic site and for the capability of DGAT1 VNTR alleles to stimulate gene expression was lacking. In the current work Sp1-VNTR interactions were analyzed by EMSA. In addition, effects of DGAT1 VNTR alleles on gene expression were measured with reporter gene analyses. Conclusions from the results are that 1) the DGAT1 VNTR sequen...Continue Reading

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Citations

Jan 28, 2012·The Journal of Dairy Research·Alfredo PauciulloLuigi Ramunno
Sep 2, 2008·Journal of Lipid Research·Chi-Liang Eric YenRobert V Farese
Dec 21, 2006·Poultry Science·A A SazanovT Malewski
May 28, 2020·Nutrients·Yarden Golan, Yehuda G Assaraf
Mar 29, 2014·Molecular Biology Reports·Izinara da Cruz RosseMaria Raquel Santos Carvalho

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