Allelic analysis of low molecular weight glutenin subunits using 2-DGE in Korean wheat cultivars

Breeding Science
Jong-Yeol LeeChoung Keun Lee

Abstract

Two-dimensional gel electrophoresis (2-DGE) was used as a complement to SDS-PAGE to determine the allelic compositions of LMW-GS in 32 Korean wheat cultivars. Protein patterns generated by 2-DGE from each cultivar were compared to patterns from standard wheat cultivars for each allele. At the Glu-A3 locus, thirteen c, twelve d, three e (null), two g and two new alleles were identified. At the Glu-B3 locus, one b, nineteen d, four h, one i and five ad alleles were identified. At the Glu-D3 locus, twenty-three a, four b, four c and one l alleles were identified. When compared to results obtained previously using SDS-PAGE, there were discrepancies in the allelic designations of 10 of 32 cultivars (31%). While SDS-PAGE is a rapid and relatively simple method for assessing LMW-GS composition, the similar mobilities of the proteins makes it difficult to discriminate certain alleles. 2-DGE is a more complicated technique, but provides a more accurate picture of the complement of the LMW-GS in a given cultivar. In addition to providing essential information for wheat breeders, the 2-DGE reference maps generated in this study will make it possible to study the contributions of individual LMW-GS to flour quality.

References

Jan 17, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W ZhangK R Gale
Nov 12, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T M IkedaH Yano
Aug 31, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·X L ZhaoW Ma
Nov 8, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·L H WangX C Xia
May 1, 1980·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P I PayneE E Mudd
Feb 18, 2016·Functional & Integrative Genomics·Jong-Yeol LeeYoung-Mi Kim

❮ Previous
Next ❯

Datasets Mentioned

BETA
B6ETS0

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Image Master Platinum

Related Concepts

Related Feeds

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.