Allelic Variation in Outer Membrane Protein A and Its Influence on Attachment of Escherichia coli to Corn Stover

Frontiers in Microbiology
Chunyu LiaoLaura R Jarboe

Abstract

Understanding the genetic factors that govern microbe-sediment interactions in aquatic environments is important for water quality management and reduction of waterborne disease outbreaks. Although chemical properties of bacteria have been identified that contribute to initiation of attachment, the outer membrane proteins that contribute to these chemical properties still remain unclear. In this study we explored the attachment of 78 Escherichia coli environmental isolates to corn stover, a representative agricultural residue. Outer membrane proteome analysis led to the observation of amino acid variations, some of which had not been previously described, in outer membrane protein A (OmpA) at 10 distinct locations, including each of the four extracellular loops, three of the eight transmembrane segments, the proline-rich linker and the dimerization domain. Some of the polymorphisms within loops 1, 2, and 3 were found to significantly co-occur. Grouping of sequences according to the outer loop polymorphisms revealed five distinct patterns that each occur in at least 5% of our isolates. The two most common patterns, I and II, are encoded by 33.3 and 20.5% of these isolates and differ at each of the four loops. Statistically signi...Continue Reading

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Citations

Feb 2, 2020·BMC Research Notes·Daniel W NielsenCatherine M Logue
Jul 22, 2018·Applied and Environmental Microbiology·Yingxi ChenLaura R Jarboe
Nov 22, 2018·Frontiers in Cellular and Infection Microbiology·Zujie YaoXiangmin Lin
Apr 5, 2021·Metabolic Engineering·Miguel C Santoscoy, Laura R Jarboe
Jul 30, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Arash MazinaniDusan Losic

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Methods Mentioned

BETA
motility agar
Protein assay
PCR
electrophoresis
flow cytometry

Software Mentioned

Hmisc
SameSpots
Weblogo
BoxPlotR
Mascot
Protein Calculator
R

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