Allelotypic and cytogenetic characterization of chemically induced mouse mammary tumors: high frequency of chromosome 4 loss of heterozygosity at advanced stages of progression

Molecular Carcinogenesis
C Marcelo AldazH Wang

Abstract

Loss of heterozygosity (LOH) is one of the most common genetic abnormalities in cancer. To define the role of LOH and chromosomal abnormalities at various stages of mouse mammary cancer progression, we analyzed the allelotypes and karyotypes of primary mammary tumors induced in CD2F, mice by two basic protocols, the classical multiple-dose 7,12-dimethylbenz[a]anthracene (DMBA) protocol and a novel protocol of combined medroxyprogesterone acetate (MPA) and DMBA. The advantage of the latter protocol is that its latency for tumor development is much shorter and its tumor incidence is higher than those of DMBA alone. To study more advanced stages of mammary tumor progression, we also analyzed mouse mammary tumors that had acquired autonomous growth and were transplantable into syngeneic hosts. The allelotypic studies were performed by means of microsatellite length polymorphism analysis with a minimum of two simple-sequence repeat markers per chromosome. We observed that MPA-DMBA-induced mammary adenocarcinomas, which in general arose earlier because of the growth promotion exerted by MPA, did not show any significant LOH and were essentially diploid. Tumors induced by DMBA alone, which on average took longer to develop, showed a h...Continue Reading

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Citations

Mar 21, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M MalumbresA Pellicer
May 5, 2010·Breast Cancer Research and Treatment·Victoria WargonClaudia Lanari
May 22, 1998·Trends in Genetics : TIG·A Balmain, H Nagase
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Apr 20, 2004·Journal of Cellular Physiology·Stella Maris VázquezIsabel Alicia Lüthy
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Feb 26, 2004·Oncogene·Duen-Hwa YanMien-Chie Hung
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S AkileshD C Roopenian
Nov 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Baocun SunYuhong Guo
Apr 30, 2003·Environmental and Molecular Mutagenesis·Fernando BenavidesLewis V Rodriguez

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