Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer

World Journal of Gastroenterology : WJG
Jing-Cui YuSong-Bin Fu

Abstract

To investigate the association between loss of heterozygosity (LOH) on chromosome 18 and sporadic gastric cancer. Multiplex PCR was used to screen 14 highly polymorphic microsatellite markers on chromosome 18 in 45 cases of primary gastric cancer. PCR products were separated on polyacrylamide gels and the electrophoresis maps were analyzed with Genescan and Genotyper. The LOH frequencies in gastric cancer at all 14 markers ranged from 10% to 58%. Eleven markers were found with over 20% LOH frequencies, in which 9 markers located in 18q, and 2 markers in 18p. Two overlapping deleted regions were identified: R1 between D18S61-D18S1161 at 18q22 (9cM) with 24% LOH frequency; R2 between D18S462-D18S70 at 18q22-23(6cM) with 32% LOH frequency. LOH of chromosome 18 (18q and 18p) may be involved in gastric tumorigenesis. Two overlapping deleted fragments suggested that there might be unidentified tumor suppressor genes in those two regions.

References

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Jul 12, 2002·Genes, Chromosomes & Cancer·Ian P M TomlinsonRebecca R Roylance
Jul 13, 2002·International Journal of Cancer. Journal International Du Cancer·Aubri L CharboneauIrene F Newsham
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Citations

Jul 31, 2014·Human Pathology·De-Bing XiangYun Wu
Oct 27, 2005·Annals of Oncology : Official Journal of the European Society for Medical Oncology·H OritaH Fujii

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